Cross-Reactive Antigens Between Pea and Some Fungal Plant Pathogens
| Autor: | G. Cristinzio, L. Coppola, Giovanni Del Sorbo, Felice Scala |
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| Přispěvatelé: | Scala, Felice, Cristinzio, Gennaro, Coppola, L., Del Sorbo, G. |
| Rok vydání: | 1994 |
| Předmět: | |
| Zdroj: | Journal of Phytopathology. 142:89-96 |
| ISSN: | 1439-0434 0931-1785 |
| Popis: | Antiserum to pea was used to analyse cross-reactive antigens (CRA) between pea and some fungal plant pathogens with different levels of specificity towards this host by using both double diffusion and immunoblotting techniques. Non pathogens of pea were also included in the study. Nectria haematococca MPVI, the three formae speciales dianthi, lycopersici and pisi of Fusarium oxysporum and Ascochyta pisi produced strong reactions with both techniques. In N. haematococca MPI, F. solani f. sp. phaseoli, V. dahliae and Phoma medicaginis var. pinodella instead, reactions were not detected by double diffusion but only the more sensitive immunoblotting technique. No CRA were observedin, the non-specific pathogens Rhizoctonia solani, Sclerotium rolfsii and Sclerotinia sclerotiorum, as well as in the non-pathogen Phytophthora capsici. The immunoblotting patterns of the most reactive fungi showed common bands with molecular weights of 84, 75 and 62 kDa. Some bands were present only in the specific pathogens N. haematococca MPVI and F. oxysporum f.sp. pisi. The possible involvement in host-parasite interactions of cross-reactive antigens which are, present in the analyzed fungi is discussed. Zusammenfassung Kreuzreaktive Antigene zwischen der Erbse und einigen pathogenen Pilzen Das erzeugte Antiserum gegen einen Extrakt aus Erbsen wurde benutzt, um die kreuzreaktiven Antigene (CRA) zwischen der Erbse und einigen zu dieser Pflanze spezifischen und nicht spezifischen pathogenen Pilzen zu analysieren. Dazu hat man sowohl die doppelte Immunostrahlungstechnik, als auch die Immunoblottingtechnik gebraucht. Nectria haematococca MPVI, die drei formae spedales dianthi, lycopersid und pisi von Fusarium oxysporum und Ascochyta pisi bewirkten die starksten |
| Databáze: | OpenAIRE |
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