Modulation of nitrate-induced phosphate response by the MYB transcription factor RLI1/HINGE1 in the nucleus
| Autor: | Stanislav Kopriva, Xiahe Huang, Junpeng Xie, Yingchun Wang, Yangwen Qian, Wei Wang, Liping Guo, Chengcai Chu, Xiujie Liu, Zhihua Zhang, Xiaohan Wang, Fanjiang Kong, Aifu Li, Shouyun Cao, Zhimin Jiang, Bin Hu, Li Zhao, Yu Yan, Baohui Liu, Legong Li, Yongqiang Liu, Yahong Qiu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Repressor Plant Science Biology 01 natural sciences Proto-Oncogene Proteins c-myb 03 medical and health sciences chemistry.chemical_compound Nitrate Gene Expression Regulation Plant medicine MYB Molecular Biology Gene Transcription factor Plant Proteins Cell Nucleus Oryza Phosphate Cell biology 030104 developmental biology medicine.anatomical_structure chemistry Cytoplasm Nucleus 010606 plant biology & botany |
| Zdroj: | Molecular Plant. 14:517-529 |
| ISSN: | 1674-2052 |
| Popis: | The coordinated utilization of nitrogen (N) and phosphorus (P) is vital for plants to maintain nutrient balance and achieve optimal growth. Previously, we revealed a mechanism by which nitrate induces genes for phosphate utilization; this mechanism depends on NRT1.1B-facilitated degradation of cytoplasmic SPX4, which in turn promotes cytoplasmic-nuclear shuttling of PHR2, the central transcription factor of phosphate signaling, and triggers the nitrate-induced phosphate response (NIPR) and N-P coordinated utilization in rice. In this study, we unveiled a fine-tuning mechanism of NIPR in the nucleus regulated by Highly Induced by Nitrate Gene 1 (HINGE1, also known as RLI1), a MYB-transcription factor closely related to PHR2. RLI1/HINGE1, which is transcriptionally activated by PHR2 under nitrate induction, can directly activate the expression of phosphate starvation-induced genes. More importantly, RLI1/HINGE1 competes with PHR2 for binding to its repressor proteins in the nucleus (SPX proteins), and consequently releases PHR2 to further enhance phosphate response. Therefore, RLI1/HINGE1 amplifies the phosphate response in the nucleus downstream of the cytoplasmic SPX4-PHR2 cascade, thereby enabling fine-tuning of N-P balance when nitrate supply is sufficient. |
| Databáze: | OpenAIRE |
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