Methanol supply speeds up synthesis gas fermentation by methylotrophic-acetogenic bacterium, Eubacterium limosum KIST612
| Autor: | Se Hwan Jang, Byeongchan Kang, Se-Hoon Park, Jiyeong Jeong, Nulee Jang, In Seop Chang, Jinsung Jeon, Jiyeon Kim, Zee-Yong Park, Mungyu Lee, Soyoung Oh |
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| Rok vydání: | 2021 |
| Předmět: |
Proteomics
0106 biological sciences Environmental Engineering Bioengineering 010501 environmental sciences 01 natural sciences chemistry.chemical_compound Bioenergy 010608 biotechnology Waste Management and Disposal 0105 earth and related environmental sciences Chromatography biology Eubacterium Renewable Energy Sustainability and the Environment Chemistry Methanol Producer gas General Medicine Metabolism biology.organism_classification Syngas fermentation Fermentation Bacteria Syngas |
| Zdroj: | Bioresource Technology. 321:124521 |
| ISSN: | 0960-8524 |
| Popis: | This study analyzed the effect of methanol on the metabolism of syngas components (i.e., H2 and CO) by the syngas fermenting acetogenic strain E. limosum KIST612. The culture characteristics and relevant proteomic expressions (as fold changes) were carefully analyzed under CO/CO2 and H2/CO2 conditions with and without methanol addition, as well as, under methanol/CO2 conditions. The culture characteristics (specific growth rate and H2 consumption rate) under H2/CO2 conditions were greatly enhanced in the presence of methanol, by 4.0 and 2.7 times, respectively. However, the promoting effect of methanol was not significant under CO/CO2 conditions. Proteomic fold changes in most enzyme expression levels in the Wood-Ljungdahl pathway and chemiosmotic energy conservation also exhibited high correspondence between methanol and H2/CO2 but not between methanol and CO/CO2. These findings suggest the advantages of methanol addition to H2/CO2 for biomass enhancement and faster consumption of gaseous substrates during syngas fermentation. |
| Databáze: | OpenAIRE |
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