Evaluation of human T cell lymphotropic virus infection in a cohort of injecting drug users
| Autor: | Ronald Altman, Steve S. Alexander, Thomas N. Denny, Carol W. Klein, Stanley H. Weiss, Bruce J. McCreedy, Paul Palumbo |
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| Rok vydání: | 1992 |
| Předmět: |
viruses
Polymerase Chain Reaction Virus Cohort Studies Antigen immune system diseases hemic and lymphatic diseases Immunology and Allergy Medicine Humans Substance Abuse Intravenous Infectivity biology New Jersey business.industry virus diseases biology.organism_classification Virology Blot Infectious Diseases Human T-lymphotropic virus 1 Human T-lymphotropic virus 2 Immunology HTLV-II Infections biology.protein Viral disease Antibody business |
| Zdroj: | The Journal of infectious diseases. 166(4) |
| ISSN: | 0022-1899 |
| Popis: | The diagnosis and confirmation of human T cell lymphotropic virus (HTLV) type II infection has proven difficult, since most assays depend on antigenic cross-reactivity between HTLV-I antigens and HTLV-II antibodies. Type-specific HTLV infection rates were evaluated in a cohort of 233 injecting drug users screened with an HTLV EIA. Of the 52 EIA-reactive specimens, 48 were indeterminate or negative by standard Western blot. Type-specific HTLV results determined by polymerase chain reaction (PCR) were 0, HTLV-I; 92%, HTLV-II; 6%, type indeterminate; and 2%, negative. Among 42 EIA-reactive, HTLV-II-PCR-positive individuals tested by a p21 envr Western blot, all were positive and 74% had antibodies to the tax protein. This study found a high rate (22.3%) of HTLV reactivity, with HTLV-II usually the sole responsible agent; shortcomings in standard HTLV-I-based diagnostics but usefulness of PCR and p21 envr Western blots for typing and confirmation of HTLV reactivity; and a high prevalence (74%) of anti-tax antibody among HTLV-II-seropositive subjects, suggesting increased potential for infectivity. |
| Databáze: | OpenAIRE |
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