Neurotransmitter-Responsive Nanosensors for T2-Weighted Magnetic Resonance Imaging
| Autor: | Santiago Recuenco Alvarado, Robert Ohlendorf, Isabel García-Álvarez, He Wei, Athena Ortega, Alan Jasanoff, Vivian Hsieh, Satoshi Okada, Jingxuan Fan, Ali Barandov |
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| Rok vydání: | 2019 |
| Předmět: |
Dopamine
Contrast Media Biosensing Techniques Conjugated system 010402 general chemistry Ligands 01 natural sciences Biochemistry Binding Competitive Catalysis Neurotransmitter binding Article chemistry.chemical_compound Colloid and Surface Chemistry Neurochemical Nanosensor medicine Animals Neurotransmitter Magnetite Nanoparticles Neurotransmitter Agents medicine.diagnostic_test Brain Magnetic resonance imaging General Chemistry Magnetic Resonance Imaging 0104 chemical sciences Rats chemistry Biophysics Serotonin medicine.drug Protein Binding |
| Zdroj: | Journal of the American Chemical Society DDFV: Repositorio Institucional de la Universidad Francisco de Vitoria Universidad Francisco de Vitoria DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria instname J Am Chem Soc |
| Popis: | Neurotransmitter-sensitive contrast agents for magnetic resonance imaging (MRI) have recently been used for mapping signaling dynamics in live animal brains, but paramagnetic sensors for T1-weighted MRI are usually effective only at micromolar concentrations that themselves perturb neurochemistry. Here we present an alternative molecular architecture for detecting neurotransmitters, using superparamagnetic iron oxide nanoparticles conjugated to tethered neurotransmitter analogs and engineered neurotransmitter binding proteins. Interactions between the nanoparticle conjugates result in clustering that is reversibly disrupted in the presence of neurotransmitter analytes, thus altering T2-weighted MRI signals. We demonstrate this principle using tethered dopamine and serotonin analogs, together with proteins selected for their ability to competitively bind either the analogs or the neurotransmitters themselves. Corresponding sensors for dopamine and serotonin exhibit target-selective relaxivity changes of up to 20%, while also operating below endogenous neurotransmitter concentrations. Semisynthetic magnetic particle sensors thus represent a promising path for minimally perturbative studies of neurochemical analytes. post-print 2482 KB |
| Databáze: | OpenAIRE |
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