The serum metabolome of COVID-19 patients is distinctive and predictive

Autor: Jifang Sheng, Silan Gu, Wenrui Wu, Yingfeng Lu, Yanfei Chen, Lanjuan Li, Jiaojiao Xie, Ren Yan, Longxian Lv, Yi-Ling Du, Ding Shi, Huiyong Jiang, Kaijin Xu, Jiafeng Xia, Jing Guo, Chenjie Huang
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Male
LC-MS
liquid chromatography-mass spectrometry
Endocrinology
Diabetes and Metabolism
CK-MB
creatine kinase-MB
Hydroxybutyrates
Butyric acid
chemistry.chemical_compound
AUC
area under the curve
0302 clinical medicine
Endocrinology
GMP
guanosine monophosphate
CRP
c-reactive protein
GGT
gamma-glutamyl transpeptidase
Prospective Studies
Amino Acids
Serum metabolome
LPV/RTV
lopinavir/ritonavir
HCs
healthy controls
COVID-19
coronavirus disease 2019
LDH
lactate dehydrogenase
TCA cycle
tricarboxylic acid cycle
Area under the curve
INR
international normalized ratio
Middle Aged
PCT
procalcitonin
Lactic acid
Cholesterol
GM3s
monosialodihexosyl gangliosides
Metabolome
Female
DB
direct bilirubin
Citric acid
Adult
medicine.medical_specialty
ALT
alanine transaminase
Predictive value
030209 endocrinology & metabolism
Fructose
SARS-CoV-2
severe acute respiratory syndrome coronavirus 2
Gas Chromatography-Mass Spectrometry
Article
WHO
World Health Organization
03 medical and health sciences
CHOL
total cholesterol
Internal medicine
PT
prothrombin time
medicine
Humans
Lactic Acid
ALB
albumin
Aged
PPARα
peroxisome proliferator-activated receptor alpha
business.industry
SARS-CoV-2
OPLS-DA
orthogonal partial least squares-discriminant analysis
COVID-19
Glutamic acid
COVID-19 Drug Treatment
Oleic acid
030104 developmental biology
chemistry
COPD
chronic obstructive pulmonary disease
HBDH
hydroxybutyrate dehydrogenase
Succinic acid
GC–MS
gas chromatography–mass spectrometry
business
Zdroj: Metabolism
ISSN: 1532-8600
0026-0495
Popis: Background Metabolism is critical for sustaining life, immunity and infection, but its role in COVID-19 is not fully understood. Methods Seventy-nine COVID-19 patients, 78 healthy controls (HCs) and 30 COVID-19-like patients were recruited in a prospective cohort study. Samples were collected from COVID-19 patients with mild or severe symptoms on admission, patients who progressed from mild to severe symptoms, and patients who were followed from hospital admission to discharge. The metabolome was assayed using gas chromatography–mass spectrometry. Results Serum butyric acid, 2-hydroxybutyric acid, l -glutamic acid, l -phenylalanine, l -serine, l -lactic acid, and cholesterol were enriched in COVID-19 and COVID-19-like patients versus HCs. Notably, d -fructose and succinic acid were enriched, and citric acid and 2-palmitoyl-glycerol were depleted in COVID-19 patients compared to COVID-19-like patients and HCs, and these four metabolites were not differentially distributed in non-COVID-19 groups. COVID-19 patients had enriched 4-deoxythreonic acid and depleted 1,5-anhydroglucitol compared to HCs and enriched oxalic acid and depleted phosphoric acid compared to COVID-19-like patients. A combination of d -fructose, citric acid and 2-palmitoyl-glycerol distinguished COVID-19 patients from HCs and COVID-19-like patients, with an area under the curve (AUC) > 0.92 after validation. The combination of 2-hydroxy-3-methylbutyric acid, 3-hydroxybutyric acid, cholesterol, succinic acid, L-ornithine, oleic acid and palmitelaidic acid predicted patients who progressed from mild to severe COVID-19, with an AUC of 0.969. After discharge, nearly one-third of metabolites were recovered in COVID-19 patients. Conclusions The serum metabolome of COVID-19 patients is distinctive and has important value in investigating pathogenesis, determining a diagnosis, predicting severe cases, and improving treatment.
Databáze: OpenAIRE
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