Role of Src Family Kinase in Extracellular Renal Cyclic Guanosine 3′,5′-Monophosphate- and Pressure-Induced Natriuresis
| Autor: | Cláudia F. Santos, Nilberto R.F. Nascimento, Robert M. Carey, Nancy L. Howell, Thurl E. Harris, Brandon A. Kemp, John J Gildea |
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| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
Natriuresis Blood Pressure Kidney Article Rats Sprague-Dawley Paracrine signalling Internal medicine Extracellular fluid Internal Medicine Extracellular medicine Animals Src family kinase Cyclic GMP Epithelial polarity Chemistry Sodium Extracellular Fluid Thionucleotides Rats Disease Models Animal src-Family Kinases Endocrinology Hypertension Female Signal transduction Signal Transduction Proto-oncogene tyrosine-protein kinase Src |
| Zdroj: | Hypertension. 58:107-113 |
| ISSN: | 1524-4563 0194-911X |
| Popis: | cGMP functions as an extracellular (paracrine) messenger acting at the renal proximal tubule and is an important modulator of pressure-natriuresis (P-N). The signaling pathway activated by cGMP in the tubule cell basolateral membrane remains unknown. We hypothesized that renal interstitial microinfusion of cGMP (50 nmol/kg per minute) or P-N would be accompanied by increased renal protein levels of phospho-Src (Tyr 416) and that the natriuresis would be decreased by Src inhibition. Renal interstitial cGMP-induced natriuresis was blocked by Src inhibitor PP2 (2.0±0.4 versus 0.5±0.01 μEq/g per minute; P P P + and lithium. Both extracellular cGMP and increased renal perfusion pressure increased renal phospho-Src protein levels and induced natriuresis in an Src-dependent manner, demonstrating that Src is an important downstream signaling molecule for extracellular cGMP-induced natriuresis. |
| Databáze: | OpenAIRE |
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