Hierarchical microchannel architecture in chitosan/bioactive glass scaffolds via electrophoretic deposition positive‐replica
| Autor: | Luigi De Nardo, Lorenza Draghi, Arash Ghalayani Esfahani, Angelica Silvia Federici, Lina Altomare, Chiara Emma Campiglio, Mehdi Soleimanzade, Aldo R. Boccaccini |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Electrophoresis
Ceramics Materials science Cell Survival Simulated body fluid 0206 medical engineering Composite number Biomedical Engineering 02 engineering and technology Matrix (biology) law.invention Biomaterials Chitosan Electrophoretic deposition chemistry.chemical_compound X-Ray Diffraction bone regeneration electrophoretic deposition (EPD) law Cell Line Tumor medicine Humans Bone regeneration Tissue Scaffolds bioactive glass 45S5 chitosan micro-structured hybrid scaffolds Metals and Alloys Settore ING-IND/34 - Bioingegneria Industriale DNA 021001 nanoscience & nanotechnology 020601 biomedical engineering Body Fluids medicine.anatomical_structure chemistry Chemical engineering Bioactive glass Thermogravimetry Ceramics and Composites Cortical bone Glass 0210 nano-technology |
| Zdroj: | Journal of Biomedical Materials Research Part A. 107:1455-1465 |
| ISSN: | 1552-4965 1549-3296 |
| DOI: | 10.1002/jbm.a.36660 |
| Popis: | One of the main challenges in the design of scaffolds for cortical bone regeneration is mimicking the highly oriented, hierarchical structure of the native tissue in an efficient, simple, and consistent way. As a possible solution to this challenge, positive replica based on electrophoretic deposition (EPD) was here evaluated as a technique to produce organic/inorganic scaffolds with oriented micro-porosities mimicking Haversian canals diameter and spacing. Two different sizes of 45S5 bioactive glass (BG) powders were chosen as inclusions and loaded in a chitosan matrix via EPD on micro-patterned cathodes. Self-standing chitosan scaffolds, with a homogeneous dispersion of BG particles and regularly-oriented micro-channels (ϕ = 380 ± 50 μm, inter-channel spacing = 600 ± 40 μm), were obtained. In vitro analysis in simulated body fluid (SBF) revealed the ability to induce a deposition of a homogenous layer of hydroxyapatite (HA), with Ca/P nucleation reactions appearing kinetically favored by smaller BG particles. Cell interaction with hybrid scaffolds was evaluated in vitro with bone osteosarcoma cells (SAOS-2). The osteoconductive potential of EPD structures was assessed by evaluating cells proliferation, viability and scaffold colonization. Results indicate that EPD is a simple yet extremely effective technique to prepare composite micro-patterned structures and can represent a platform for the development of a new generation of bone scaffolds. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2019. |
| Databáze: | OpenAIRE |
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