Neuroprotective Effects of a Standardized Flavonoid Extract from Safflower against a Rotenone-Induced Rat Model of Parkinson’s Disease
| Autor: | Deyong Lv, Min Ye, Nuramatjan Ablat, Xin Zhao, Yingcong Ma, Hongbin Han, Rutong Ren, Yilixiati Xiaokaiti, Jiamin Xu, Feng Xu, Xian-Rong Qi, Hui Lei, Yi Sun, Xiang Ma, Xiao-Ping Pu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Parkinson's disease DA Carthamus tinctorius Pharmaceutical Science Substantia nigra Pharmacology Neuroprotection Article Analytical Chemistry lcsh:QD241-441 Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine lcsh:Organic chemistry Dopamine Rotenone Drug Discovery medicine Animals Parkinson Disease Secondary Physical and Theoretical Chemistry Dopamine transporter Flavonoids biology Tyrosine hydroxylase Plant Extracts safflower flavonoid extract Parkinson’s disease MRI Organic Chemistry Dopaminergic medicine.disease Rats Neuroprotective Agents 030104 developmental biology Biochemistry chemistry Chemistry (miscellaneous) biology.protein Molecular Medicine 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Molecules Molecules, Vol 21, Iss 9, p 1107 (2016) Molecules; Volume 21; Issue 9; Pages: 1107 |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules21091107 |
| Popis: | Parkinson’s disease (PD) is a major age-related neurodegenerative disorder characterized by the loss of dopaminergic neurons in the substantia nigra par compacta (SNpc). Rotenone is a neurotoxin that is routinely used to model PD to aid in understanding the mechanisms of neuronal death. Safflower (Carthamus tinctorius. L.) has long been used to treat cerebrovascular diseases in China. This plant contains flavonoids, which have been reported to be effective in models of neurodegenerative disease. We previously reported that kaempferol derivatives from safflower could bind DJ-1, a protein associated with PD, and that a flavonoid extract from safflower exhibited neuroprotective effects in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced mouse model of PD. In this study, a standardized safflower flavonoid extract (SAFE) was isolated from safflower and found to primarily contain flavonoids. The aim of the current study was to confirm the neuroprotective effects of SAFE in rotenone-induced Parkinson rats. The results showed that SAFE treatment increased body weight and improved rearing behavior and grip strength. SAFE (35 or 70 mg/kg/day) treatment reversed the decreased protein expression of tyrosine hydroxylase, dopamine transporter and DJ-1 and increased the levels of dopamine and its metabolite. In contrast, acetylcholine levels were decreased. SAFE treatment also led to partial inhibition of PD-associated changes in extracellular space diffusion parameters. These changes were detected using a magnetic resonance imaging (MRI) tracer-based method, which provides novel information regarding neuronal loss and astrocyte activation. Thus, our results indicate that SAFE represents a potential therapeutic herbal treatment for PD. |
| Databáze: | OpenAIRE |
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