The effect of vascular complications of diabetes mellitus on human umbilical cord tissue and the number of Wharton Jelly's mesenchymal stem cells

Autor: Cigdem Karaca, Demet Tasdemir Kahraman, Nuray Bostancieri, Ali Ovayolu
Rok vydání: 2020
Předmět:
0301 basic medicine
medicine.medical_specialty
Cord
Cell Count
GPI-Linked Proteins
Gastroenterology
Umbilical cord
Fetal Macrosomia
Umbilical Cord
03 medical and health sciences
0302 clinical medicine
Pregnancy
Internal medicine
Diabetes mellitus
Wharton's jelly
Genetics
medicine
Umbilical Cord Diameter
Humans
Diabetic Nephropathies
Wharton Jelly
Molecular Biology
5'-Nucleotidase
Cells
Cultured
business.industry
Mesenchymal stem cell
Endoglin
Infant
Newborn
Mesenchymal Stem Cells
General Medicine
Arteries
medicine.disease
Immunohistochemistry
Gestational diabetes
Diabetes
Gestational
030104 developmental biology
medicine.anatomical_structure
Hyaluronan Receptors
030220 oncology & carcinogenesis
Case-Control Studies
Thy-1 Antigens
Female
business
Diabetic Angiopathies
Artery
Follow-Up Studies
Zdroj: Molecular biology reports. 47(12)
ISSN: 1573-4978
Popis: The current study investigated the change in umbilical cord tissue and the number of markers of Wharton’s jelly mesenchymal stem cells (WJ-MSC) in pregnant women with gestational diabetes (GDM), with chronic diabetes who developed nephropathy as vascular complication (VC-PGDM), and healthy pregnant women as the control. The umbilical cords (UC) were investigated by the histomorphological method and the number of WJ-MSC were detected by flow-cytometry using the CD90, CD44, CD105, and CD73 markers in Wharton’s jelly (WJ) isolated from fresh umbilical cords. The number of positive cells for CD 90, CD44, CD105, and CD73 were found to be elevated in the GDM group, whereas it was significantly diminished in the VC-PGDM group (p = 0.001, p = 0.001, p = 0.001, and p = 0.001). The only histopathological sign in the GDM group were an increased number of pores in the Wharton jelly. Artery wall thickness/cord diamater ratio was increased, which indicates an increase of the artery wall thickness in the VC- PGDM group (p = 0.039 and p = 0.048). The increase in umbilical cord diameter and number of Wharton jelly mesenchymal stem cells in babies of gestational diabetic mothers was considered as an effect of macrosomia seen in babies of mothers with gestational diabetes. Vasculopathy, a long-term complication of diabetes, is known to affect all tissues by causing marked lower perfusion and hypoxia, as well as a decrease in the MSC number in our study.
Databáze: OpenAIRE
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