Seroprevalence of Toxocara infection in children from southern Brazil
| Autor: | Alan J. A. McBride, Carlos James Scaini, Sibele Borsuk, Marcos Marreiro Villela, Elizandra Roselaine Schoenardie, Claudiomar Soares Brod, Maria Elizabeth Aires Berne, Michele Soares Pepe |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Veterinary medicine Blotting Western Antibodies Helminth Enzyme-Linked Immunosorbent Assay Biology Dogs Western blot Visceral larva migrans Seroepidemiologic Studies medicine Confidence Intervals Odds Ratio Seroprevalence Animals Humans Child Ecology Evolution Behavior and Systematics Toxocariasis medicine.diagnostic_test Age Factors Infant Toxocara canis biology.organism_classification medicine.disease Canis Antigens Helminth Child Preschool Immunology biology.protein Parasitology Female Antibody Brazil |
| Zdroj: | The Journal of parasitology. 99(3) |
| ISSN: | 1937-2345 |
| Popis: | The seroprevalence of Toxocara canis antibodies in children aged from 1 to 12 yr old was evaluated in Pelotas City, Rio Grande do Sul, Brazil. Human toxocariasis or visceral larva migrans (VLM) was diagnosed with the use of an ELISA based on the T. canis excretory-secretory (TES) antigens; Western blotting was used to confirm the ELISA-positive results. From 427 samples, 50.6% were positive for the presence of anti-TES antibodies. A confirmatory test (Western blot) was carried out on a sample of the ELISA- positive sera (n ¼ 70), and all were positive. The Western blots had specific banding pattern characteristics, where the 30-kDa fraction demonstrated the highest reactivity. This fraction could be important for the specific diagnosis of toxocariasis. |
| Databáze: | OpenAIRE |
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