Multiple hormone actions: The rises in cAMP and Ca ++ in mdck-cells treated with glucagon and prostaglandin E 1 are independent processes
| Autor: | Nicol P. Kurstjens, Fritz Boege, Richard C. Cantrill, Helmuth Heithier, Matthias Hahn |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Agonist
medicine.medical_specialty Indoles medicine.drug_class medicine.medical_treatment Biophysics Fluorescence spectrometry Adenylate kinase chemistry.chemical_element Calcium Kidney Biochemistry Cyclase Glucagon Cell Line Receptors Gastrointestinal Hormone chemistry.chemical_compound Dogs 1-Methyl-3-isobutylxanthine Internal medicine Cyclic AMP Receptors Glucagon medicine Animals Alprostadil Molecular Biology Fluorescent Dyes Forskolin Colforsin Isoproterenol Cell Biology Kinetics Endocrinology chemistry Carbachol Prostaglandin E |
| Zdroj: | Biochemical and Biophysical Research Communications. 167:1162-1169 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(90)90645-4 |
| Popis: | Glucagon and prostaglandin E 1 stimulate adenylate cyclase in Madin-Darby canine kidney cells with an approximate EC 50 of 3 ∗ 10 −8 and 1 ∗ 10 −7 M respectively. The rise in cAMP is accompanied by a transient rise in intracellular Ca ++ measured with the fluorescent calcium indicator Indo-1. A comparable increase in intracellular Ca 2+ without a rise in cAMP occurs with the cholinergic agonist carbamylcholine. Stimulation of adenylate cyclase by the β-adrenergic agonist isoproterenol or directly by forskolin has no effect on intracellular Ca ++ . By all criteria studied the rise in intracellular Ca ++ and the increase in cAMP are independent from each other. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|