A green analytical assay for the quantitation of the total saponins in quinoa (Chenopodium quinoa Willd.) based on macro lens-coupled smartphone
| Autor: | Sarela Alfaro-Cruz, Noemí León-Roque, Wile Mamani-Navarro, Silvana Aguilar-Tuesta, Luis Condezo-Hoyos, Juan Quispe-Neyra |
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| Rok vydání: | 2019 |
| Předmět: |
Resolution (mass spectrometry)
Relative standard deviation Saponin 02 engineering and technology 01 natural sciences Chenopodium quinoa Chemistry Techniques Analytical Analytical Chemistry Limit of Detection Animals Bovine serum albumin Edetic Acid chemistry.chemical_classification Chromatography biology 010401 analytical chemistry Reproducibility of Results Green Chemistry Technology Serum Albumin Bovine Saponins 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Seeds Correlation analysis Micro plate biology.protein Cattle Smartphone 0210 nano-technology |
| Zdroj: | Talanta. 204:576-585 |
| ISSN: | 0039-9140 |
| DOI: | 10.1016/j.talanta.2019.06.014 |
| Popis: | The aim of this study was to develop and validate a microtiter macro lens-coupled smartphone (MCS) assay for the quantitation of the total saponins in quinoa based on foam measurement. The 96-well micro plate with a black bottom and an inclination angle = 12.102° of macro lens-coupled smartphone allows to acquire images with a high resolution. The foam stability, a critical aspect for the MCS assay, was significantly improved by the inclusion of a chelating agent (EDTA 50 mmol L −1 ) and bovine serum albumin (0.5 mg mL −1 ). The MCS assay was linear within the range of 9.039–180.773 × 10 −4 mg mL −1 of saponin (R 2 = 0.9929), using the integrated density/area as a foam measurement (Y) and the logarithm of saponin concentration (X) (Y = 372.1 + 104.2LogX). The MCS assay was 50-folds more sensitive than afrosimetric assay -AA- with LOD = 3.168 × 10 −4 mg mL −1 and LOQ = 4.784 × 10 −4 mg mL −1 . MCS assay was more reproducible (relative standard deviation (RSD) = 0.632–9.646%) than AA (RSD = 3.44–44.04%). The correlation analysis, Bland-Altman analysis and Passing-Bablok regression showed good agreement between total saponin content in quinoa as measured by the MCS assay and AA. Based on the green analytical procedure index, MCS assay can be considered as a green procedure. |
| Databáze: | OpenAIRE |
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