High-performance liquid chromatography of lactose with evaporative light scattering detection, applied to determine fine particle dose of carrier in dry powder inhalation products
| Autor: | Heribert Häusler, Peter Langguth, Peter Grass, Bianca Beilmann |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Detection limit
Chromatography Light Chemistry Hydrophilic interaction chromatography Organic Chemistry Extraction (chemistry) Analytical chemistry Reproducibility of Results Lactose General Medicine Biochemistry High-performance liquid chromatography Light scattering Analytical Chemistry Solvent Chromatography detector Administration Inhalation Scattering Radiation Particle Powders Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography A. 1107:204-207 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2005.12.083 |
| Popis: | A method for quantification of the fine particle dose of lactose is described, using a hydrophilic interaction chromatography (HILIC) method and evaporative light scattering detection. The HILIC method used an aminopropyl column and a mobile phase consisting of acetonitril/water (80/20, v/v) for isocratic elution. Sensitive chromatography was obtained using a low concentration of water in the extraction solvent. The detection limit (RSD10%) at an injection volume of 10 microL is 10 microg/mL. Linearity was obtained in the range of 10-80 microg/mL (R(2)0.99). A relative standard deviation (RSD) of 0.5% (N=6) demonstrated good precision of the optimized method. |
| Databáze: | OpenAIRE |
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