Isolation of atropine and scopolamine from plant material using liquid-liquid extraction and EXtrelut® columns
| Autor: | Anna Topolewska, Artur Maciejka, Łukasz P. Haliński, Piotr Stepnowski, Paula Śramska |
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| Rok vydání: | 2017 |
| Předmět: |
Atropine
Datura stramonium Chromatography Gas Liquid-Liquid Extraction Scopolamine Clinical Biochemistry Ethyl acetate 01 natural sciences Biochemistry Analytical Chemistry Sonication chemistry.chemical_compound Limit of Detection Liquid–liquid extraction medicine Hyoscyamine Chromatography biology Plant Extracts 010405 organic chemistry Chemistry 010401 analytical chemistry Extraction (chemistry) Reproducibility of Results Tropane Cell Biology General Medicine biology.organism_classification Diatomaceous Earth 0104 chemical sciences Datura Linear Models Gas chromatography medicine.drug |
| Zdroj: | Journal of Chromatography B. 1043:202-208 |
| ISSN: | 1570-0232 |
| Popis: | Tropane alkaloids are toxic secondary metabolites produced by Solanaceae plants. Among them, plants from Datura genus produce significant amounts of scopolamine and hyoscyamine; the latter undergoes racemization to atropine during isolation. Because of their biological importance, toxic properties and commonly reported food and animal feed contamination by different Datura sp. organs, there is a constant need for reliable methods for the analysis of tropane alkaloids in many matrices. In the current study, three extraction and sample-clean up procedures for the determination of scopolamine and atropine in plant material were compared in terms of their effectiveness and repeatability. Standard liquid-liquid extraction (LLE) and EXtrelut® NT 3 columns were used for the sample clean-up. Combined ultrasound-assisted extraction and 24h static extraction using ethyl acetate, followed by multiple LLE steps was found the most effective separation method among tested. However, absolute extraction recovery was relatively low and reached 45-67% for atropine and 52-73% for scopolamine, depending on the compound concentration. The same method was also the most effective one for the isolation of target compounds from Datura stramonium leaves. EXtrelut® columns, on the other hand, displayed relatively low effectiveness in isolating atropine and scopolamine from such a complex matrix and hence could not be recommended. The most effective method was also applied to the extraction of alkaloids from roots and stems of D. stramonium. Quantitative analyses were performed using validated method based on gas chromatography with flame ionization detector (GC-FID). Based on the results, the importance of the proper selection of internal standards in the analysis of tropane alkaloids was stressed out. |
| Databáze: | OpenAIRE |
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