The small GTP-binding protein rac regulates growth factor-induced membrane ruffling
| Autor: | Caroline L. Johnston, Dagmar Diekmann, Hugh Paterson, Alan Hall, Anne J. Ridley |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Membrane ruffling
Growth factor medicine.medical_treatment Rac3 Rho family of GTPases RhoGAP domain RAC1 Endogeny Cell Biology Biology General Biochemistry Genetics and Molecular Biology Cell biology Rac GTP-Binding Proteins biology.protein medicine RhoG Signal transduction Cytoskeleton Microinjection Actin |
| Zdroj: | Trends in Cell Biology. 2:324 |
| ISSN: | 0962-8924 |
| Popis: | The function of rac, a ras-related GTP-binding protein, was investigated in fibroblasts by microinjection. In confluent serum-starved Swiss 3T3 cells, rac1 rapidly stimulated actin filament accumulation at the plasma membrane, forming membrane ruffles. Several growth factors and activated H-ras also induced membrane ruffling, and this response was prevented by a dominant inhibitory mutant rac protein, N17rac1. This suggests that endogenous rac proteins are required for growth factor-induced membrane ruffling. In addition to membrane ruffling, a later response to both rac1 microinjection and some growth factors was the formation of actin stress fibers, a process requiring endogenous rho proteins. Using N17rac1 we have shown that these growth factors act through rac to stimulate this rho-dependent response. We propose that rac and rho are essential components of signal transduction pathways linking growth factors to the organization of polymerized actin. |
| Databáze: | OpenAIRE |
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