Phasic, Nonsynaptic GABA-A Receptor-Mediated Inhibition Entrains Thalamocortical Oscillations
| Autor: | Péter Barthó, Zita Rovó, Anita Lüthi, Csaba Dávid, Chiara Pellegrini, Andrea Slézia, Simone Astori, Balázs Hangya, Sandro Lecci, Ferenc Mátyás, László Acsády |
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| Rok vydání: | 2014 |
| Předmět: |
Thalamus
Mice Transgenic Sleep spindle Biology Tonic (physiology) GABA Antagonists Mice 03 medical and health sciences Bursting 0302 clinical medicine Animals Receptor gamma-Aminobutyric Acid 030304 developmental biology Cerebral Cortex Neurons 0303 health sciences GABAA receptor General Neuroscience Neural Inhibition Articles Dependovirus Receptors GABA-A Mice Inbred C57BL Pyridazines Inhibitory Postsynaptic Potentials nervous system Synapses Reticular connective tissue Vesicular Glutamate Transport Protein 2 Cerebral Cortex/physiology Dependovirus/genetics Excitatory Amino Acid Antagonists/pharmacology GABA Antagonists/pharmacology Inhibitory Postsynaptic Potentials/drug effects Inhibitory Postsynaptic Potentials/genetics Neural Inhibition/physiology Neurons/physiology Pyridazines/pharmacology Receptors GABA-A/genetics Receptors GABA-A/metabolism Synapses/drug effects Synapses/genetics Thalamus/physiology Vesicular Glutamate Transport Protein 2/metabolism gamma-Aminobutyric Acid/metabolism GABAergic Excitatory Amino Acid Antagonists Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | JOURNAL OF NEUROSCIENCE Journal of Neuroscience, vol. 34, no. 21, pp. 7137-7147 |
| DOI: | 10.1523/JNEUROSCI.4386-13.2014 |
| Popis: | GABA-A receptors (GABA-ARs) are typically expressed at synaptic or nonsynaptic sites mediating phasic and tonic inhibition, respectively. These two forms of inhibition conjointly control various network oscillations. To disentangle their roles in thalamocortical rhythms, we focally deleted synaptic, γ2 subunit-containing GABA-ARs in the thalamus using viral intervention in mice. After successful removal of γ2 subunit clusters, spontaneous and evoked GABAergic synaptic currents disappeared in thalamocortical cells when the presynaptic, reticular thalamic (nRT) neurons fired in tonic mode. However, when nRT cells fired in burst mode, slow phasic GABA-AR-mediated events persisted, indicating a dynamic, burst-specific recruitment of nonsynaptic GABA-ARs.In vivo, removal of synaptic GABA-ARs reduced the firing of individual thalamocortical cells but did not abolish slow oscillations or sleep spindles. We conclude that nonsynaptic GABA-ARs are recruited in a phasic manner specifically during burst firing of nRT cells and provide sufficient GABA-AR activation to control major thalamocortical oscillations. |
| Databáze: | OpenAIRE |
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