Enzymatic studies of urinary isomeric chondroitin sulfates from patients with mucopolysaccharidoses. The application of high performance liquid chromatography
| Autor: | James E. Evans, George Jia-Long Lee, John T. Dulaney, Howard Tieckelmann, Edwin W. Naylor |
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| Rok vydání: | 1980 |
| Předmět: |
Chromatography
Chondroitin Sulfates Biochemistry (medical) Clinical Biochemistry Disaccharide General Medicine Urine Mucopolysaccharidoses Biochemistry High-performance liquid chromatography Dermatan sulfate Chondroitinases and Chondroitin Lyases carbohydrates (lipids) Glycosaminoglycan chemistry.chemical_compound Sulfation Isomerism chemistry Humans Chondroitin Chondroitin sulfate Chromatography High Pressure Liquid Glycosaminoglycans |
| Zdroj: | Clinica Chimica Acta. 104:65-75 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/0009-8981(80)90135-7 |
| Popis: | The high-performance liquid chromatographic (HPLC) method for the determination of unsaturated sulfated disaccharides is a comprehensive and reliable method which expedites ensymatic studies of isomeric chondroitin sulfates. Responses for these unsaturated disaccharides derived from urinary chondroitin sulfates were linear from 100 ng to 10 micrograms injected and good quantitation was obtained for 25 microliters or less of samples placed on the column. This method which is more sensitive and accurate than methods now being used has considerable potential for the chemical diagnosis of patients with mucopolysaccharidoses and for the clarification of glycosaminoglycan structure. The isomeric chondroitin sulfates in urines from patients with mucopolysaccharidoses were studied by enzyme digestion with chondroitinases followed by HPLC determination of the sulfated unsaturated disaccharides produced. Evaluation by HPLC of the unsaturated 4-sulfated disaccharide produced by digestion of the urinary GAG with chondroitinases ABC and AC revealed rapidly and quantitatively the large amounts of dermatan sulfate present in Hurler, Hunter, and Maroteaux-Lamy urines. Chondroitin 4-sulfate predominated in Sanfilippo urinary isomeric chondroitin sulfates whereas chondroitin 6-sulfate and chondroitin 4-sulfate were shown to be present in nearly equal amounts in Morquio urine. An oversulfated chondroitin sulfate was detected in small amounts in some of these urines. This was demonstrated by the detection of an unsaturated disulfated disaccharide after digestion with chondroitinase ABC but not with chondroitinase AC. |
| Databáze: | OpenAIRE |
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