Pheno- and genotypic features of Epstein-Barr virus associated B-Cell lymphoproliferations in peripheral T-Cell lymphomas
| Autor: | Balázs Márton, Ágnes Lacza, László Pajor, Katalin Keresztes, Zsófia Nagy, Gabor Smuk, László Kereskai, Árpád Illés |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Cancer Research
Epstein-Barr Virus Infections Lymphoma B-Cell CD30 Genotype T cell Population Biology medicine.disease_cause Gene Rearrangement T-Lymphocyte Klinikai orvostudományok Polymerase Chain Reaction Pathology and Forensic Medicine Diagnosis Differential immune system diseases hemic and lymphatic diseases medicine T-cell lymphoma Humans education Gene Rearrangement B-Lymphocyte B cell In Situ Hybridization education.field_of_study B-Lymphocytes Lymphoma T-Cell Peripheral Receptors Antigen T-Cell gamma-delta General Medicine Gene rearrangement Orvostudományok medicine.disease Epstein–Barr virus Virology Molecular biology Immunohistochemistry Lymphoma medicine.anatomical_structure Phenotype Oncology RNA Viral Immunoglobulin Heavy Chains |
| Popis: | Among the 300 peripheral T-cell lymphomas (PTCL) searched for EBV positive non-resting B-cells by EBER in situ hybridization 12 have been identified with various forms of EBV-driven B-cell proliferation. This could be categorized into three major forms. i. In the first form scattered immature, mononuclear B-cells of immuno-, centroblastic type with CD20+. CD30+ CD45+, LMP1+ phenotype, reactive appearance and polyclonal immunoglobulin heavy chains gene rearrangement (IgH-R) were admixed to the PTCL cells. ii. The second form mimicked diffuse large B-cell lymphoma as homogenous sheets, largely demarcated from the PTCL, of mononuclear, immature B-cell of CD20+, CD30+, CD45+, LMP1+, EBNA-2+ phenotype but with lack of monoclonal IgH-R were present. iii. In the third form scattered Hodgkin-Reed-Sternberg (HRS) type of cells were noticed which exhibited the CD15+/-, CD20-/+, CD30+, CD45-, LMP1+, EBNA-2- phenotype and in 50% showed clonal IgH gene rearrangement in whole tissue DNA extract. The IgH associated transcription factors' (OCT2, BOB.1/OBF.1, PU.1) expression patterns in these cells corresponded to those of HRS cells in cHL. Based on analysis of 65 PTCLs, we have identified in the positive cases a highly significant increase of EBV+ small, reactive, resting B-cell compartment (75.9 / 100 HPF in PTCL vs. 1.5 / 100 HPF in control lymph nodes) likely to be due to the decreased immune surveillance. This progressive accumulation of EBV+ by-stander B-cell population in PTCLs might be the source of various B-cell proliferations, which in any form represent major diagnostic pitfalls and require a careful differential diagnostic procedure. |
| Databáze: | OpenAIRE |
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