Effect ofEscherichia coliendotoxin onArchachatina marginatahemolymph coagulation system

Autor:	Adenike Temidayo Oladiji, Musa Toyin Yakubu, O. B. Oloyede, Musa Oyewole Salawu, N. O. Muhammad
Rok vydání:	2011
Předmět:	Hemocytes animal structures Lysis Snails Drug Evaluation Preclinical Pharmaceutical Science Biology medicine.disease_cause Microbiology Plasma Hemolymph Drug Discovery Escherichia coli medicine Animals Blood Coagulation Limulus Test Pharmacology Archachatina marginata Biological Products Hemostasis Hemolymph coagulation Inoculation Proteins General Medicine Sterilization (microbiology) biology.organism_classification Endotoxins Disease Models Animal Complementary and alternative medicine Rabbit test Molecular Medicine Rabbits
Zdroj:	Pharmaceutical Biology. 49:1029-1033
ISSN:	1744-5116 1388-0209
DOI:	10.3109/13880209.2011.560952
Popis:	Archachatina marginata Swainson (Achatinidae) is found in Nigeria, West Africa. Its hemolymph is applied as a disinfectant to blades and fresh cuts of circumcision in Yorubaland. The hemolymph is also used in traditional medicine practice. Investigation into its anti-endotoxin response is being studied for the first time.This study determined whether endotoxin causes measurable and concentration-dependent protein coagulation in the separate hemolymph fractions and in hemocyte lysate (HL)/plasma mixtures.Endotoxin was prepared by inoculating 5% w/v dextrose with locally isolated Escherichia coli cells and incubated for 48 h before sterilization. Pyrogenicity was determined by rabbit test method and use the of LAL kit. Hemolymph fractions were exposed to endotoxin while controls were exposed to endotoxin-free water (0.025 EU/ml). HL/plasma (1:1 v/v) was exposed to varied endotoxin concentrations.Data indicated significantly higher protein coagulates induced by endotoxin in all the hemolymph fractions (P0.05). Maximum protein coagulation in mixture of HL/plasma 1:1 was recorded. Exposure of HL/plasma at optimal ratio to varied endotoxin caused linear protein coagulation up to 1.0 EU/ml, beyond which it dropped significantly and unresponsive to further increase in endotoxin doses.There was endotoxin-induced protein coagulation, which is endotoxin concentration-dependent. The optimal coagulation observed for 1:1 HL/plasma mixture suggests stronger interaction between the hemocytes and the plasma in response to endotoxin. There are LPS-binding proteins in the plasma and hemocytes of A. marginata. This finding may be employed in detection and quantification of endotoxin in future.
Databáze:	OpenAIRE
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