Effect of bedside filtration on aggregates from cold-stored whole blood-derived platelet-rich plasma and apheresis platelet concentrates
| Autor: | Jill Corson, Lydia Fang, Barbara A. Osborne, Valery J Li, Chomkan Usaneerungrueng, Moritz Stolla, Jeffrey Miles, S. Lawrence Bailey, José A López, Tahsin Özpolat, Yanyun Wu |
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| Rok vydání: | 2021 |
| Předmět: |
Blood Platelets
medicine.medical_specialty Hematology Chemistry Platelet-Rich Plasma Immunology Phlebotomy law.invention Andrology Cold Temperature Platelet transfusion Apheresis law Blood Preservation Internal medicine Platelet-rich plasma medicine Blood Component Removal Immunology and Allergy Humans Platelet Filtration Whole blood |
| Zdroj: | TransfusionREFERENCES. 62(1) |
| ISSN: | 1537-2995 |
| Popis: | Background The current approach to manufacture cold-stored platelets (CSP) replicates that of room temperature-stored platelets (RSP). However, this production method is associated with aggregate formation in CSP, a major pitfall that leads to significant wastage. We hypothesized that isolating platelets from whole blood as platelet-rich plasma (PRP) and storing them at a lower concentration reduces aggregates and that conventional bedside transfusion filtration removes CSP aggregates. Methods We collected platelets from healthy humans by apheresis (AP) and by phlebotomy, from which we generated platelet-rich plasma (PRP). We split each AP and PRP platelets into two equal aliquots, storing one at 22°C (RT-PRP and RT-AP) and the other at 4°C (4C-PRP and 4C-AP). We evaluated platelets on day 0 and day 7 of storage. After storage, we measured platelet counts, aggregates, and other key characteristics before and after filtration by a bedside filter. Results After storage, the 4C-AP platelet counts decreased significantly. 4C-PRP preserved glucose better and prevented a significant increase in lactate contrary to 4C-AP. Filtration led to significantly lower platelet counts in both 4C-PRP and 4C-AP but not in their RT counterparts. Post filtration, we observed 50% fewer aggregates only in 4C-AP, whereas 4C-PRP showed an unexpected but significant increase in aggregates. Testing confirmed activation during storage but filtration did not further activate platelets. Conclusion We provide evidence that 4C-PRP is an alternative to 4C-AP and that bedside filters reduce aggregates from 4C-AP. Further studies are needed to evaluate the hemostatic potential of 4C-PRP and the management of aggregates. |
| Databáze: | OpenAIRE |
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