Keratinocytes-Derived Reactive Oxygen Species Play an Active Role to Induce Type 2 Inflammation of the Skin: A Pathogenic Role of Reactive Oxygen Species at the Early Phase of Atopic Dermatitis
| Autor: | M. Piao, Seung-Chul Lee, Jun-Hyeong Park, Jee-Bum Lee, Da-In Choi, Min-Song Suh, Jee-Young Choi, Sook-Jung Yun |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Keratinocytes
Thymic stromal lymphopoietin Inflammation Dermatology Pathogenesis House dust mite 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine medicine Atopic dermatitis chemistry.chemical_classification Reactive oxygen species Neurogenic inflammation biology business.industry Interleukin Type 2 inflammation of the skin medicine.disease biology.organism_classification chemistry 030220 oncology & carcinogenesis Immunology Original Article medicine.symptom business |
| Zdroj: | Annals of Dermatology |
| ISSN: | 2005-3894 1013-9087 |
| Popis: | Background: Atopic dermatitis (AD) is characterized by chronic, relapsing skin inflammation (eczema) with itchy sensation. Keratinocytes, which are located at the outermost part of our body, are supposed to play important roles at the early phase of type 2 inflammation including AD pathogenesis. Objective: The purpose of this study was to evaluate whether keratinocytes-derived reactive oxygen species (ROS) could be produced by the allergens or non-allergens, and the keratinocytes- derived ROS could modulate a set of biomarkers for type 2 inflammation of the skin. Methods: Normal human epidermal keratinocytes (NHEKs) were treated with an allergen of house dust mites (HDM) or a non-allergen of compound 48/80 (C48/80). Then, biomarkers for type 2 inflammation of the skin including those for neurogenic inflammation were checked by reverse transcriptase-polymerase chain reaction and western immunoblot experiments. Results: HDM or C48/80 was found to upregulate expression levels of our tested biomarkers, including type 2 T helper-driving pathway (KLK5, PAR2, and NFκB), epithelial-cell-derived cytokines (thymic stromal lymphopoietin, interleukin [IL]-25, IL-33), and neurogenic inflammation (NGF, CGRP). The HDMor C-48/80-induced expression levels of the biomarkers could be blocked by an antioxidant treatment with 5 mM N-acetyl- cysteine. In contrast, pro-oxidant treatment with 1 mM H2O2 could upregulate expression levels of the tested biomarkers in NHEKs. Conclusion: Our results reveal that keratinocytes- derived ROS, irrespective to their origins from allergens or non-allergens, have a potential to induce type 2 inflammation of AD skin. (Ann Dermatol 33(1) 26∼36, 2021) |
| Databáze: | OpenAIRE |
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