The gastroprotective effect of pogostone from Pogostemonis Herba against indomethacin-induced gastric ulcer in rats
Autor: | Jian-Hui Xie, Haiming Chen, Yi-Feng Zheng, Lu-Di Fu, Yong-Zhuo Liang, Yu-Hong Liu, Xiao-Ping Lai, Zu-Qing Su, Xiao-Ying Chen, Zi-Ren Su, Xie Zhang, Zhen-Biao Zhang, Xiao-Qi Huang |
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Rok vydání: | 2015 |
Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Indomethacin Lansoprazole Administration Oral Pharmacology Ulcer index Severity of Illness Index General Biochemistry Genetics and Molecular Biology Superoxide dismutase Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Gastrointestinal Agents Oral administration Gastric mucosa Oils Volatile Medicine Animals Humans Stomach Ulcer Prostaglandin E2 Original Research Gastrointestinal agent Microscopy biology business.industry Histocytochemistry Glutathione Immunohistochemistry Disease Models Animal 030104 developmental biology medicine.anatomical_structure Treatment Outcome chemistry Gastric Mucosa 030220 oncology & carcinogenesis biology.protein business medicine.drug |
Zdroj: | Experimental biology and medicine (Maywood, N.J.). 241(2) |
ISSN: | 1535-3699 |
Popis: | Pogostemonis Herba, known as “Guang-Huo-Xiang” in Chinese, has been widely used in the treatment of gastrointestinal dysfunction. Pogostone is one of the major constituents of Pogostemonis Herba. The aim was to scientifically evaluate the possible gastroprotective effect and the underlying mechanisms of pogostone against indomethacin-induced gastric ulcer in rats. Rats were orally treated with vehicle, lansoprazole (30 mg/kg) or pogostone (10, 20 and 40 mg/kg) and subsequently exposed to acute gastric lesions induced by indomethacin. Gross evaluation, histological observation, gastric mucosal superoxide dismutase activity, glutathione content, catalase activity, malonaldehyde level and prostaglandin E2 production were performed. Immunohistochemistry and reverse transcription polymerase chain reaction for cyclooxygenase-1 and cyclooxygenase-2, as well as terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling assay, immunohistochemistry for heat-shock protein 70, B-cell lymphoma-2 and Bax were conducted. Results indicated that rats pretreated with pogostone showed remarkable protection from the gastric mucosa damage compared to vehicle-treated rats based on the ulcer index and inhibition percentage. Histologically, oral administration of pogostone resulted in observable improvement of gastric injury, characterized by reduction of necrotic lesion, flattening of gastric mucosa and alleviation of submucosal edema with hemorrhage. Pogostone pretreatment significantly raised the depressed activities of superoxide dismutase, glutathione and catalase, while reduced the elevated malonaldehyde level compared with indomethacin-induced group. Pogostone-pretreated group induced a significant increase in gastric mucosal prostaglandin E2 level and obvious up-regulation of protein levels and mRNA expressions of cyclooxygenase-1 and cyclooxygenase-2. Furthermore, antiapoptotic effect of pogostone was verified by terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling assay, and the apoptotic process triggered by pogostone involved the up-expression of heat-shock protein70 and B-cell lymphoma-2 protein, and suppression of Bax protein expressions in the ulcerated tissues. It is speculated that the gastroprotective effect of pogostone against indomethacin-induced gastric ulceration might be associated with its stimulation of cyclooxygenase-mediated prostaglandin E2, antioxidant and antiapoptotic effect. |
Databáze: | OpenAIRE |
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