Separation of Bovine Spermatozoa by Density on Water Insoluble Newtonian Gels and Their Use for Insemination
| Autor: | R J Wall, Anthony R. Zine, Albert August Luderer, R H Foote, Donna M. Hess, W W Dean |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Male
endocrine system medicine.medical_treatment Fraction (chemistry) Semen Cell Separation Water insoluble Biology Insemination Centrifugation Density Gradient medicine Animals Sex Ratio reproductive and urinary physiology Chromatography urogenital system Artificial insemination Sperm density Cell Biology General Medicine Anatomy Silicone Gels Spermatozoa Reproductive Medicine Sperm Motility Cattle Acrosome Gels Semen Preservation Specific gravity |
| Zdroj: | Biology of Reproduction. 26:813-824 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod26.5.813 |
| Popis: | Bovine spermatozoa were separated into different density subpopulations utilizing water insoluble hydrocarbon and silicone gels of defined specific gravity. Sperm density profiles were generated for 13 bulls. The separations were found to be repeatable and characteristic of the bull examined. Considerable density variation among animals was demonstrated. Analysis of the separated spermatozoa, before and after freezing, demonstrated that good motility and aerosomal integrity of spermatozoa were maintained. When the least dense fraction of spermatozoa was used for insemination, conception rates were similar to those obtained routinely by artificial insemination with unfractionated spermatozoa. Therefore, this system may be useful in separating spermatozoa of various densities and for removing extraneous matter from semen. However, the sex ratio, among 51 60-day-old fetuses recovered from heifers inseminated with the lowest density fraction of spermatozoa, was 26 males: 25 females. |
| Databáze: | OpenAIRE |
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