| Autor: |
Tan, Yi-Zhou, Xu, Xin-Yue, Dai, Ji-Min, Yin, Yuan, He, Xiao-Tao, Zhang, Yi-Lin, Tian-Xiao Zhu, An, Ying, Bei-Min Tian, Chen, Fa-Ming |
| Rok vydání: |
2021 |
| DOI: |
10.6084/m9.figshare.14516624.v1 |
| Popis: |
Additional file 1: Supplementary Fig. 1. Isolation and identification of human PDLSCs. Human PDLSCs were cultured and passaged in vitro; across the in vivo expansion stage, passage 2 (P2), passage 7 (P7), and passage 15 (P15) cells were collected for the following examinations. (a) Expression of cell surface markers in P2, P7 and P15 cells as determined by flow cytometry. (b) Chondrogenic differentiation in P2, P7 and P15 cells according to Alcian blue staining (representative images, scale bar = 100 μm). (c) Adipogenic differentiation in P2, P7 and P15 cells according to Oil Red O staining (representative images of lipid droplets, scale bar = 100 μm). (d) Osteogenic differentiation potential of P2, P7 and P15 cells as indicated by Alizarin Red staining (top: representative general views; bottom: representative images of calcium nodules, scale bar = 100 μm). (e) Quantitative analysis of Alizarin Red staining for P2, P7 and P15 cells (* p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
