Mechanotransductive cascade of Myo-II-dependent mesoderm and endoderm invaginations in embryo gastrulation
| Autor: | Frédéric Dumas-Bouchiat, Laurent Ranno, Aude Michel, Nora M. Dempsey, Jens-Christian Röper, Christine Ménager, Gorky Shaw, Démosthène Mitrossilis, Simon Le Denmat, Damien Le Roy, Emmanuel Farge, Benjamin Driquez |
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| Přispěvatelé: | Laboratoire Physico-Chimie Curie [Institut Curie] (PCC), Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Micro et NanoMagnétisme (NEEL - MNM), Institut Néel (NEEL), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), PHysicochimie des Electrolytes et Nanosystèmes InterfaciauX (PHENIX), Université Pierre et Marie Curie - Paris 6 (UPMC)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Optique & Microscopies (NEEL - POM), HAL UPMC, Gestionnaire, Mechanics and Genetics of Embryonic Development Group, PSL Research University, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Micro et NanoMagnétisme (MNM ), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Optique et microscopies (POM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Curie [Paris]-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Mesoderm Embryo Nonmammalian Science [SDV]Life Sciences [q-bio] General Physics and Astronomy Biology Mechanotransduction Cellular Article General Biochemistry Genetics and Molecular Biology Magnetics 03 medical and health sciences medicine Animals Mechanotransduction Process (anatomy) Myosin Type II Multidisciplinary Endoderm Gastrulation Gene Expression Regulation Developmental Embryo General Chemistry Embryonic stem cell Cell biology [SDV] Life Sciences [q-bio] Drosophila melanogaster 030104 developmental biology medicine.anatomical_structure RNA Interference NODAL |
| Zdroj: | Nature Communications Nature Communications, 2017, 8, pp.13883. ⟨10.1038/ncomms13883⟩ Nature Communications, Nature Publishing Group, 2017, 8, pp.13883. ⟨10.1038/ncomms13883⟩ Nature Communications, Vol 8, Iss 1, Pp 1-15 (2017) |
| ISSN: | 2041-1723 |
| Popis: | Animal development consists of a cascade of tissue differentiation and shape change. Associated mechanical signals regulate tissue differentiation. Here we demonstrate that endogenous mechanical cues also trigger biochemical pathways, generating the active morphogenetic movements shaping animal development through a mechanotransductive cascade of Myo-II medio-apical stabilization. To mimic physiological tissue deformation with a cell scale resolution, liposomes containing magnetic nanoparticles are injected into embryonic epithelia and submitted to time-variable forces generated by a linear array of micrometric soft magnets. Periodic magnetically induced deformations quantitatively phenocopy the soft mechanical endogenous snail-dependent apex pulsations, rescue the medio-apical accumulation of Rok, Myo-II and subsequent mesoderm invagination lacking in sna mutants, in a Fog-dependent mechanotransductive process. Mesoderm invagination then activates Myo-II apical accumulation, in a similar Fog-dependent mechanotransductive process, which in turn initiates endoderm invagination. This reveals the existence of a highly dynamic self-inductive cascade of mesoderm and endoderm invaginations, regulated by mechano-induced medio-apical stabilization of Myo-II. Mechanical signals regulate tissue differentiation but how this triggers downstream biochemical signals is unclear. Here, the authors place micro-magnets in the Drosophila embryonic epithelia and show this triggers apical pulsations, in turn stabilizing Myosin-II, resulting in mesoderm invagination. |
| Databáze: | OpenAIRE |
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