The effects of cobalt protoporphyrin IX and tricarbonyldichlororuthenium (II) dimer treatments and its interaction with nitric oxide in the locus coeruleus of mice with peripheral inflammation
Autor: | Moreno, Patricia, Cazuza, R. A., Gomes-Mendes, J., Díaz, A. F., Polo, S., Leánez, Sergi, Leite-Panissi, C. R. A., Pol, Olga., Universitat Autònoma de Barcelona |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
MAPK/ERK pathway
Male Inflammatory pain Nitric Oxide Synthase Type II Protoporphyrins Nitric Oxide Synthase Type I Pharmacology medicine.disease_cause lcsh:Chemistry chemistry.chemical_compound Mice Locus coeruleus Carbon monoxide lcsh:QH301-705.5 Spectroscopy inflammatory pain Mice Knockout ESTRESSE OXIDATIVO Heme oxygenase 1 Chemistry Kinase analgesia General Medicine COPP Computer Science Applications medicine.symptom heme oxygenase 1 NOS1 Inflammation Nitric Oxide Catalysis Article carbon monoxide Nitric oxide Inorganic Chemistry medicine Organometallic Compounds Animals Physical and Theoretical Chemistry Molecular Biology locus coeruleus Organic Chemistry Heme oxygenase lcsh:Biology (General) lcsh:QD1-999 Analgesia Oxidative stress Biomarkers |
Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 20, Iss 9, p 2211 (2019) Volume 20 Issue 9 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau instname Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona |
ISSN: | 1422-0067 |
Popis: | Heme oxygenase 1 (HO-1) and carbon monoxide were shown to normalize oxidative stress and inflammatory reactions induced by neuropathic pain in the central nervous system, but their effects in the locus coeruleus (LC) of animals with peripheral inflammation and their interaction with nitric oxide are unknown. In wild-type (WT) and knockout mice for neuronal (NOS1-KO) or inducible (NOS2-KO) nitric oxide synthases with inflammatory pain induced by complete Freund&rsquo s adjuvant (CFA), we assessed: 1) antinociceptive actions of cobalt protoporphyrin IX (CoPP), an HO-1 inducer 2) effects of CoPP and tricarbonyldichlororuthenium(II)dimer (CORM-2), a carbon monoxide-liberating compound, on the expression of HO-1, NOS1, NOS2, CD11b/c, GFAP,and mitogen-activated protein kinases (MAPK)in the LC. CoPP reduced inflammatory pain in different time-dependent manners in WT and KO mice. Peripheral inflammation activated astroglia in the LC of all genotypes and increased the levels of NOS1 and phosphorylated extracellular signal-regulated kinase 1/2 (p-ERK 1/2) in WT mice. CoPP and CORM-2 enhanced HO-1 and inhibited astroglial activationin all genotypes. Both treatments blocked NOS1 overexpression,and CoPP normalized ERK 1/2 activation. This study reveals an interaction between HO-1 and NOS1/NOS2 during peripheral inflammation andshows that CoPP and CORM-2 improved HO-1 expression and modulated the inflammatory and/or plasticity changes caused by peripheral inflammation in the LC. |
Databáze: | OpenAIRE |
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