Production of isotope-labeled proteins in insect cells for NMR
| Autor: | Alvar D. Gossert, Shin Isogai, Leonildo Delgado, Anne Grahl, Christian Opitz, Stephan Grzesiek, Bastian Franke |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Insecta Biochemistry law.invention 03 medical and health sciences law Animals Humans Yeast extract Prokaryotic expression Nuclear Magnetic Resonance Biomolecular Spectroscopy G protein-coupled receptor chemistry.chemical_classification Insect cell Chemistry Proteins Nuclear magnetic resonance spectroscopy Recombinant Proteins Yeast Amino acid 030104 developmental biology Isotope Labeling Recombinant DNA Baculoviridae |
| Zdroj: | Journal of Biomolecular NMR. 71:173-184 |
| ISSN: | 1573-5001 0925-2738 |
| Popis: | Baculovirus-infected insect cells have become a powerful tool to express recombinant proteins for structural and functional studies by NMR spectroscopy. This article provides an introduction into the insect cell/baculovirus expression system and its use for the production of recombinant isotope-labeled proteins. We discuss recent advances in inexpensive isotope-labeling methods using labeled algal or yeast extracts as the amino acid source and give examples of advanced NMR applications for proteins, which have become accessible by this eukaryotic expression host. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink