Inhibition of the β-catenin/Tcf signaling by caffeoylquinic acids in sweet potato leaf through down regulation of the Tcf-4 transcription
Autor: | Eito Tsuchida, Junsei Taira, Masatsugu Uehara, Wakana Ohmine |
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Rok vydání: | 2013 |
Předmět: |
Colorectal cancer
Quinic Acid Down-Regulation Biology chemistry.chemical_compound Transcription Factor 4 Downregulation and upregulation Transcription (biology) Caffeic acid medicine Humans Ipomoea batatas beta Catenin Catechol Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Plant Extracts Wnt signaling pathway food and beverages General Chemistry medicine.disease HCT116 Cells Plant Leaves Caffeoylquinic acid chemistry Biochemistry Polyphenol General Agricultural and Biological Sciences Signal Transduction Transcription Factors |
Zdroj: | Journal of agricultural and food chemistry. 62(1) |
ISSN: | 1520-5118 |
Popis: | Sweet potato leaves contain the highest levels of functional polyphenols. In this study the effects of the sweet potato leaf extract and its contents, such as mono (3, 4, and 5)-caffeoylquinic acid (CQA), di-CQA (4,5-diCQA, 3,5-diCQA, and 3,4-diCQA) and caffeic acid (CA), were evaluated on the β-catenin/Tcf-4 signaling in human colorectal cancer HCT116 cells. The extract and the CQA derivatives inhibited the β-catenin/Tcf-4 signaling, and the inhibition of the di-CQA (with two caffeoyl groups) was higher than that of the mono-CQA (one-caffeoyl group) and CA, suggesting that the caffeoyl structure in the presence of a catechol group plays a significant role in interfering with the β-catenin/Tcf-4 signaling. In addition, the CQA derivatives had no effect on the β-catenin protein expression, but all test compounds inhibited the expression of the Tcf-4 transcription, and the inhibition of the di-CQA derivatives was stronger than those of the mono-CQA derivatives as well as the β-catenin/Tcf-4 transcriptional activity. These compounds can modulate the downstream Wnt signaling pathway, suggesting that sweet potato leaves can be a protective food for colorectal cancer. |
Databáze: | OpenAIRE |
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