Whole blood microvolume laser scanning cytometry for monitoring resting and activated platelets
| Autor: | Timothy L. Wyant, Patrick C. Smith, Brad Brown, Aaron B. Kantor |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Blood Platelets
Pathology medicine.medical_specialty CD36 Platelet Membrane Glycoproteins Specimen Handling Antigen Antigens CD medicine Humans Platelet Platelet activation Whole blood biology Platelet Count Chemistry Lasers Anticoagulants Hematology General Medicine Flow Cytometry Platelet Activation Molecular biology Laser Scanning Cytometry biology.protein Antibody CD61 Biomarkers |
| Zdroj: | Platelets. 12:309-318 |
| ISSN: | 1369-1635 0953-7104 |
| DOI: | 10.1080/09537100120068206 |
| Popis: | Enumerating and phenotyping of platelets, resting and activated, from whole blood is important for both the identification and verification of many disease states. Microvolume laser scanning cytometry (MLSC) has been shown to be a simple method for enumerating and phenotyping peripheral blood cells. Here, the utility of MLSC, in conjunction with an anticoagulant containing platelet activation inhibitors, for simultaneously measuring platelet count, phenotype and responsiveness directly from non-fixed whole blood was examined. CTAD or EDTA anticoagulated blood was collected from five to 20 healthy volunteers, stained with fluorescence-labeled antibodies specific for platelet antigens, and run on an in-house modified MSLC device. MLSC was able to measure antigens CD9, CD29, CD36, CD41, CD42a, CD42b, and CD61 on platelets and determine an average of 2.3 x 10(5) +/- 7 x 10(4) platelets per microliter. Counts correlated well with those obtained from the Cell-Dyn 3500 (r(2)=0.84). Agreeing with previous data, less than 2% of platelets from peripheral blood of normal individuals expressed the activation markers CD62P or CD63. After in vitro thrombin activation,93% of the platelets expressed activation markers. Data presented here shows the benefits of using MLSC in combination with platelet inhibitors to quantitate and phenotype platelets while maintaining a viable responsive state. |
| Databáze: | OpenAIRE |
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