DETERMINATION OF BIOFILM FORMING ACTIVITY OF MICROORGANISMS ON SYNTHETIC POLYMERIC MATERIALS
| Autor: | M S Stepanov, M .V. Yakovlev, A P Godovalov, K. A. Batog, E E Kobzarenko |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
Dental composite Materials science Escherichia coli K12 Polyurethanes Biochemistry (medical) Composite number Biofilm Glass ionomer cement Dental Cements General Medicine Polymer Medical Laboratory Technology chemistry.chemical_compound Polyvinyl chloride chemistry Glass Ionomer Cements Biofilms Candida albicans Polystyrene Zinc Oxide Composite material Polyvinyl Chloride Polyurethane |
| Zdroj: | Russian Clinical Laboratory Diagnostics. 64:758-761 |
| ISSN: | 0869-2084 |
| DOI: | 10.18821/0869-2084-2019-64-12-758-761 |
| Popis: | Microorganisms are able to form biofilms on surfaces of biotic and abiotic nature. In turn, in human biotopes there are optimal conditions for the implementation of biofilm-forming activity. Moreover, in medical practice, polymeric materials are often used for drainage or prosthetics, which can also be successfully colonized by bacteria. However, in laboratory practice, the formation of biofilms is usually evaluated on glass or polystyrene. The purpose of the study is to evaluate the methodological features of studying the biofilm-forming activity of microorganisms on the surface of synthetic polymeric materials. We used strains of Staphylococcus aureus ATCC 25923, Escherichia coli K-12, Candida albicans ATCC 10231, as well as synthetic polymeric materials - DentLight Flow light-curing composite material (nano-hybrid fluid composite; Russia), glass ionomer chemical curing Fuji 1 (Japan), cement for temporary fixation of orthopedic constructions TempBond NE (USA), acrylic, polyurethane and polyvinyl chloride. The formation of biofilms in flat-bottomed ELISA plates in this study was considered as a control group. If the polymer belonged to cold curing materials, sterile flat-bottomed tablets were used, the bottom of which was filled with a thin layer of plastic. After hardening of the plastic, biofilms were formed in the tablets. In the second series of experiments, hot cured materials cut into equal parts 5×5×1 mm in size were placed in the wells of a plate and again used to determine biofilm formation with subsequent coloring. To extract the dye, the pieces were transferred to a new plate to exclude the amount of film biomass formed on the walls of the plate wells. In both cases, cultivation was carried out at 37° C for 24-48 hours. The biomass of the film was stained with fuchsin. Statistical data processing was performed using t-Student criterion. For the threshold level of significance, the value p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|