Galanin promotes neuronal differentiation in murine subventricular zone cell cultures

Autor: David P.D. Woldbye, Tiago Santos, Raquel Ferreira, Thomas Harnois, Liliana Bernardino, Laura Sardà-Arroyo, Nicolas Bourmeyster, Fabienne Agasse, José Bragança, Alexandra Isabel Rosa, João O. Malva, Søren H. Christiansen, Clarissa S. Schitine, Sara Xapelli, Valérie Coronas
Rok vydání: 2013
Předmět:
medicine.medical_specialty
Tyrosine 3-Monooxygenase
Vesicular Inhibitory Amino Acid Transport Proteins
animal diseases
Neurogenesis
Neuronal differentiation
Cell Culture Techniques
Subventricular zone
Galanin
Nerve Tissue Proteins
Brain damage
Biology
Cerebral Ventricles
Mice
Neural Stem Cells
Cell Movement
Internal medicine
medicine
Basic Helix-Loop-Helix Transcription Factors
Animals
Receptor
Cells
Cultured
Cell Proliferation
Neurons
Cell Death
Nuclear Proteins
Cell Differentiation
Cell Biology
Hematology
Cell movement
Neural stem cell
Peptide Fragments
Receptor
Galanin
Type 1
Cell biology
Receptor
Galanin
Type 2
DNA-Binding Proteins
Mice
Inbred C57BL
Endocrinology
medicine.anatomical_structure
nervous system
Cell culture
medicine.symptom
Microtubule-Associated Proteins
Developmental Biology
Zdroj: Stem cells and development. 22(11)
ISSN: 1557-8534
Popis: Neural stem cells of the subventricular zone (SVZ) represent a potentially important source of surrogate cells for the treatment of brain damage. Proper use of these cells for neuronal replacement depends on the ability to drive neuronal differentiation. Several neuromodulators stimulate neurogenesis. Here we examined the effects of the neuropeptide galanin, on neuronal differentiation in murine SVZ cultures. SVZ neurospheres obtained from early postnatal mice were treated with 10 nM to 2 μM galanin. Galanin promoted neuronal differentiation, increasing numbers of NeuN-, vesicular GABA transporter- and tyrosine hydroxylase-expressing neurons. In contrast, galanin neither affected cell proliferation assessed by BrdU incorporation nor cell death evaluated by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL). Neuronal differentiation was further confirmed at the functional level by measuring [Ca(2+)]i variations in single SVZ cells after KCl and histamine stimulations to distinguish neurons from immature cells. Galanin treatment increased the numbers of neuronal-like responding cells compared to immature cells. Using selective agonists (M617, AR-M1896) and antagonists (galantide, M871) for galanin receptors 1 and 2, we showed that both galanin receptors mediated neuronal differentiation. Early proneuronal effects of galanin included positive regulation of the transcription factor neurogenin-1 (Ngn1). In addition, galanin promoted axonogenesis and dendritogenesis, increasing both the length of phosphorylated stress-activated protein kinase- and Tau-positive axons and the numbers of microtubule associated protein-2 (MAP-2)-labelled dendrites. Moreover, galanin inhibited SVZ cell migration in the transwell assay. Our results show a proneurogenic effect of galanin and open new perspectives for future applications in stem cell-based therapies for neuronal replacement.
Databáze: OpenAIRE
Externí odkaz: