Leading-process actomyosin coordinates organelle positioning and adhesion receptor dynamics in radially migrating cerebellar granule neurons
Autor: | Ryan A. Kerekes, Niraj Trivedi, Joseph S. Ramahi, Mahmut Karakaya, Danielle Howell, David J. Solecki |
---|---|
Rok vydání: | 2014 |
Předmět: |
Motility
Golgi Apparatus macromolecular substances Biology 03 medical and health sciences symbols.namesake Mice 0302 clinical medicine Developmental Neuroscience Cell Movement Cerebellum Organelle Cell polarity Myosin Animals Actin 030304 developmental biology Myosin Type II Neurons 0303 health sciences Cilium Cell Polarity Actomyosin Golgi apparatus Actins Cell biology Mice Inbred C57BL Platelet Glycoprotein GPIb-IX Complex Centrosome symbols 030217 neurology & neurosurgery Research Article |
Zdroj: | Neural Development |
ISSN: | 1749-8104 |
Popis: | Background During brain development, neurons migrate from germinal zones to their final positions to assemble neural circuits. A unique saltatory cadence involving cyclical organelle movement (e.g., centrosome motility) and leading-process actomyosin enrichment prior to nucleokinesis organizes neuronal migration. While functional evidence suggests that leading-process actomyosin is essential for centrosome motility, the role of the actin-enriched leading process in globally organizing organelle transport or traction forces remains unexplored. Results We show that myosin ii motors and F-actin dynamics are required for Golgi apparatus positioning before nucleokinesis in cerebellar granule neurons (CGNs) migrating along glial fibers. Moreover, we show that primary cilia are motile organelles, localized to the leading-process F-actin-rich domain and immobilized by pharmacological inhibition of myosin ii and F-actin dynamics. Finally, leading process adhesion dynamics are dependent on myosin ii and F-actin. Conclusions We propose that actomyosin coordinates the overall polarity of migrating CGNs by controlling asymmetric organelle positioning and cell-cell contacts as these cells move along their glial guides. Electronic supplementary material The online version of this article (doi:10.1186/1749-8104-9-26) contains supplementary material, which is available to authorized users. |
Databáze: | OpenAIRE |
Externí odkaz: |