Yeast artificial chromosome segregation from host chromosomes with similar lengths
| Autor: | Vadim V. Demidov, Nikolay O. Bukanov, Maxim D. Frank-Kamenetskii, Konstantin I. Izvolsky |
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| Rok vydání: | 1998 |
| Předmět: |
Yeast artificial chromosome
Peptide Nucleic Acids congenital hereditary and neonatal diseases and abnormalities Saccharomyces cerevisiae Genetic Vectors chemical and pharmacologic phenomena Genome chemistry.chemical_compound hemic and lymphatic diseases Genetics Humans Genomic library Cloning Molecular DNA Fungal Chromosomes Artificial Yeast Gene Library biology fungi hemic and immune systems biology.organism_classification Yeast Electrophoresis Gel Pulsed-Field genomic DNA chemistry Chromosomes Fungal DNA Cytokinesis Research Article |
| Zdroj: | Nucleic acids research. 26(21) |
| ISSN: | 0305-1048 |
| Popis: | We propose a new method for segregation of yeast artificial chromosomes (YACs) from endogenous yeast chromosomes with similar lengths. The method is based on recently developed PNA-assisted rare cleavage (PARC) of genomic DNA. We apply the PARC procedure to YAC-containing samples of yeast DNA in such a way that host chromosomes, which electrophoretically comigrate with the chosen YACs, are selectively digested while YACs remain intact. These data demonstrate that a pool of appropriate PNAs can be used as an efficient tool for the PARC-based isolation of intact purified YACs directly from the host cells. |
| Databáze: | OpenAIRE |
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