Standardized Peptidome Profiling of Human Serum for the Detection of Pancreatic Cancer
| Autor: | Edgar Zapico-Muñiz, Nayra Rico-Santana, Antoni Farré-Viladrich, Josefina Mora-Brugués, Francesc González-Sastre |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Proteomics Oncology medicine.medical_specialty serum peptide profiling Adolescent CA-19-9 Antigen Endocrinology Diabetes and Metabolism pancreatic cancer Sensitivity and Specificity Ca 19 9 antigen Young Adult Endocrinology Internal medicine Pancreatic cancer Biomarkers Tumor Internal Medicine medicine MALDI-TOF MS Humans Young adult Reference standards Aged Tumor marker standardization Aged 80 and over Hepatology business.industry biomarkers Reproducibility of Results Middle Aged Reference Standards Serum samples medicine.disease Pancreatic Neoplasms Logistic Models CA 19-9 Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Pancreatitis Female Peptides business |
| Zdroj: | PANCREAS r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau instname |
| ISSN: | 0885-3177 |
| DOI: | 10.1097/mpa.0b013e3181dfcbe5 |
| Popis: | Objective: To establish new biomarkers for accurate diagnosis of pancreatic cancer (PC) using a standardized serum peptidome profiling and compare the results with those from the tumor marker, CA 19-9. Methods: Serum samples from 102 patients (55 with chronic pancreatitis and 47 with PC) and 56 healthy controls were collected and analyzed following a protocol that was rigorously designed to prevent preanalytical variation. Serum peptides were extracted using immobilized copper ion chromatography on a robotic platform. Mass spectra were acquired by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry on an Autoflex II spectrometer (Bruker Daltonics, Bremen, Germany). Statistical analysis was performed using the Clinprotools 2.2 software (Bruker Daltonics) and the SPSS 15.0 software (SPSS Inc, Chicago, III). Results: Standardized peptidome profiling showed a median coefficient of variation of 11.6% calculated using all the extracted peptides and negligible influence of sex and age on peptidome profiles. The diagnostic sensitivity was 89.9%, and the diagnostic specificity was 92.7%, using 2 serum features and CA 19-9 serum concentration. Healthy controls were differentiated from patients with PC and chronic pancreatitis, with the use of 3 features of the peptidome (diagnostic sensitivity, 98.2%; diagnostic specificity, 97.1%). Conclusions: Standardized serum peptidome profiling could be a useful tool to improve biochemical diagnosis of PC in combination with the classic tumor marker, CA 19-9. |
| Databáze: | OpenAIRE |
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