| Autor: |
Wallach, Thomas, Mossmann, Zo�� J., Szczepek, Michal, Wetzel, Max, Machado, Rui, Raden, Martin, Miladi, Milad, Kleinau, Gunnar, Kr��ger, Christina, Dembny, Paul, Adler, Drew, Zhai, Yuanyuan, Kumbol, Victor, Dzaye, Omar, Sch��ler, Jutta, Futschik, Matthias, Backofen, Rolf, Scheerer, Patrick, Lehnardt, Seija |
| Rok vydání: |
2021 |
| DOI: |
10.6084/m9.figshare.17088338 |
| Popis: |
Additional file 5. Low dose response of miR-100-5p- and miR-298-5p-treated microglia and HEK293 hTLR7/8 reporter cells. (a) Images of C57BL/6 microglia incubated with 5 ��g/ml of miR-100-5p or miR-298-5p, or PBS (control) for 4 h. Subsequently, cells were fixed and immunolabeled with Iba1 antibody, while nuclei were visualized with DAPI. Compared to control, miRNA-treated microglia displayed an amoeboid morphology, indicating an activated state. Scale bar, 30 ��m. (b) Microglia were incubated with indicated doses of miR-100-5p or miR-298-5p for 24 h. Loxoribine (1 mM) and LPS (100 ng/ml) served as positive control. Unstimulated condition served as negative control. Subsequently, supernatants were analyzed by TNF-�� ELISA. Data are represented as mean �� SD, n = 3. (c) HEK-Blue cells co-expressing human TLR7 (left, center) or human TLR8 (right), and an NF-��B/AP1-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene were incubated with various doses of miR-100-5p or miR-298-5p, as indicated, for 24 h. Loxoribine (1 mM), R848 (100 ng/ml), or TNF-�� (100 ng/ml) served as positive control. Unstimulated HEK-Blue TLR-expressing cells and HEK-Blue Null1 or Null1-k cells served as negative control. Data are expressed as fold change of optical density of the SEAP protein normalized to unstimulated control. Data are represented as mean �� SD, n = 3. *P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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