Low level 809-nm diode laser-induced in vitro stimulation of the proliferation of human gingival fibroblasts
| Autor: | Bernd d'Hoedt, A.B. Christoffers, Brita Willershausen, Haitham Al-Haj, Matthias Kreisler |
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| Rok vydání: | 2002 |
| Předmět: |
Pathology
medicine.medical_specialty business.industry Lasers medicine.medical_treatment Gingiva Connective tissue Dermatology Fibroblasts In Vitro Techniques Statistics Nonparametric Andrology Tissue culture medicine.anatomical_structure Relative fluorescence units Cell culture medicine Humans Surgery Irradiation Fibroblast business Cells Cultured Low level laser therapy Explant culture |
| Zdroj: | Lasers in Surgery and Medicine. 30:365-369 |
| ISSN: | 1096-9101 0196-8092 |
| DOI: | 10.1002/lsm.10060 |
| Popis: | Background and Objective The authors investigated the effects of low level laser irradiation on the proliferation rate of human gingival fibroblasts (HGF) in vitro. Study Design/Materials and Methods HGF were obtained from gingival connective tissue explants and cultured under standard conditions. 110 cell cultures in their logarithmic growth phase were spread on 96-well tissue culture plates and were irradiated at energy fluences of 1.96–7.84 J/cm2. Another 110 cultures served as control. An 809-nm semiconductor laser operated at a power output of 10 mW in the cw-mode was used. The time of exposure varied between 75 and 300 seconds. Laser treatment was performed alternatively once, twice, and three times at a 24-hour interval. After lasing, incubation was continued for 24 hours. The proliferation rate was determined by means of fluorescence activity of a redox indicator added to the cell culture. Proliferation was determined 24, 48, and 72 hours after irradiation and expressed in relative fluorescence units (RFU). Results The irradiated cells revealed a considerably higher proliferation activity. The differences were highly significant 24 hour after irradiation (Mann-Whitney U-test, P |
| Databáze: | OpenAIRE |
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