Expression of adenylyl cyclase type I in cochlear inner hair cells
| Autor: | Ali H Shakir, Kirk W. Beisel, Steve Kim, Ahmad A Karadaghy, James S. Hatfield, Khalid M. Khan, Roberto L. Barretto, John M Lasak, Dennis G. Drescher, Andrew J Drescher, Marian J. Drescher |
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| Rok vydání: | 1997 |
| Předmět: |
DNA
Complementary Transcription Genetic Stereocilia (inner ear) education Molecular Sequence Data chemistry.chemical_element Calcium Biology Polymerase Chain Reaction Adenylyl cyclase Cellular and Molecular Neuroscience chemistry.chemical_compound Mice otorhinolaryngologic diseases medicine Animals Inner ear RNA Messenger Receptor Molecular Biology Organ of Corti Cochlea DNA Primers Gene Library Hair Cells Auditory Inner Base Sequence Molecular biology Rats Rats Inbred ACI medicine.anatomical_structure chemistry Mice Inbred CBA sense organs Hair cell Adenylyl Cyclases |
| Zdroj: | Brain research. Molecular brain research. 45(2) |
| ISSN: | 0169-328X |
| Popis: | Expression of calcium/calmodulin-activated adenylyl cyclase type I (ACI) mRNA has been determined in the cochlea and in an organ-of-Corti subdissected tissue fraction by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Amplification products of predicted size were obtained from the mouse cochlea and rat organ of Corti with nucleotide sequences corresponding to respective ACI brain transcripts. In addition, ACI template was detected in a rat inner hair cell cDNA library by PCR. Immunoreactivity to ACI has been localized within the organ of Corti to the inner hair cell, with diaminobenzidine staining found in both the cell body and in the stereocilia. Evidence, thus, has been obtained that both ACI transcript and protein are expressed in the inner hair cell, the primary mechanosensory receptor cell of the cochlea. We hypothesize that ACI is activated by calcium influx through a calcium/calmodulin interaction and that this adenylyl cyclase isoform may have a role in modulation of receptoneural afferent transmission and/or mechanosensory transduction in the cochlea. © 1997 Elsevier Science B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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