In Vitro Topographical Model of Fuchs Dystrophy for Evaluation of Corneal Endothelial Cell Monolayer Formation
| Autor: | Alon R. Mendez, Muhammad Rizwan, Evelyn K.F. Yim, Khadijah Adnan, Sacha L. Naso, Jodhbir S. Mehta, Gary S L Peh |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Corneal endothelium Pathology medicine.medical_specialty medicine.medical_treatment Biomedical Engineering Pharmaceutical Science Biology Fuchs' dystrophy Cell Line Cornea Biomaterials Cell therapy 03 medical and health sciences 0302 clinical medicine Monolayer medicine Humans Corneal transplantation Endothelium Corneal Fuchs' Endothelial Dystrophy Endothelial Cells Dystrophy Epithelial Cells Cell migration Anatomy medicine.disease eye diseases Endothelial stem cell 030104 developmental biology 030221 ophthalmology & optometry sense organs |
| Zdroj: | Advanced Healthcare Materials. 5:2896-2910 |
| ISSN: | 2192-2640 |
| DOI: | 10.1002/adhm.201600848 |
| Popis: | A common indication for corneal transplantation, which is the most transplanted tissue, is a dysfunctional corneal endothelium due to Fuchs' endothelial dystrophy (FED). FED is diagnosed by the presence of in vivo pathological microtopography on the Descemet membrane, which is called corneal guttata. Minimally invasive corneal endothelial cell regenerative procedures such as endothelial cell injection therapy and Rho kinase inhibitor pharmacotherapy have been proposed as alternatives to conventional corneal transplantation for FED patients. However, the effect of guttata on monolayer reformation following such therapies is unknown and there is no equivalent in vitro or animal model to study monolayer reformation. Using a synthetic guttata FED disease model, the formation of the monolayer is investigated to evaluate the efficacy of both therapies. Results obtained suggest that guttata dimensions, density, and spacing greatly affect the fate of corneal endothelial cells in terms of migratory behavior and monolayer reformation. Densely packed synthetic guttata mimicking late-stage FED hinders monolayer reformation, while synthetic guttata of lower height and density show improved monolayer formation. These results suggest that severity of the FED, as determined by height and density of existing guttata, can potentially attenuate corneal endothelial monolayer formation of corneal cell injection therapy and pharmacotherapy. |
| Databáze: | OpenAIRE |
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