Comparative Study of Glucose Transporters GLUT-2 and GLUT-5 in Ostriches Gastrointestinal Tract
| Autor: | Tõnu Järveots, Piret Hussar, Lazo Pendovski, Ilmars Duritis, Florina Popovska-Percinic, Martin Kärner |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Gastrointestinal tract medicine.medical_specialty lcsh:Veterinary medicine GLUT-5 General Veterinary GLUT-2 040301 veterinary sciences Glucose transporter Physiology ostriches 04 agricultural and veterinary sciences Biology Gastroenterology 0403 veterinary science 03 medical and health sciences 030104 developmental biology Internal medicine medicine lcsh:SF600-1100 gastrointestinal tract |
| Zdroj: | Macedonian Veterinary Review, Vol 39, Iss 2, Pp 225-231 (2016) |
| ISSN: | 1857-7415 |
| DOI: | 10.1515/macvetrev-2016-0089 |
| Popis: | The knowledge about transport of sugars in animals and birds gastrointestinal tract is very important for science as carbohydrates are the main energy source of food. Since until now there is few information about the localization of glucose transporters - integral membrane proteins that mediate the transport of glucose and related substances across the cellular membranes - in birds gastrointestinal tract, the aim of the present study was to localize glucose transporters-2 and -5 (GLUT-2 and -5) in three parts of the ostriches gastrointestinal tract – proventriculus, duodenum and ileum - comparatively in ostrich chicken in their early ontogenesis period. Material from the superficial gland zone of the proventriculus, duodenum and terminal zone of the ileum were collected from eight female ostriches (Struthio camelus var. Domesticus): two chickens after hatching, three 7 and three 30-days old ostriches. The material was fixed with 10% formalin, embedded into paraffin, slices 7 μm thick were cut followed by immunohistochemical staining with polyclonal primary antibodies Rabbit anti-GLUT-2 and Rabbit anti-GLUT-5, carried out according to the manufacturers guidelines (IHC kit, Abcam, UK). The results showed that the staining for both antibodies was weaker in all parts of the gastrointestinal tract of ostriches after hatching compared to 7 and 30 days old ostriches showing that the gastrointestinal tract of ostriches immediately after hatching is not entirely capable of transportation of carbohydrates. The results of our study may indicate the possibility of a close relationship between feeding and the ability to transport sugars in the gastrointestinal tract. |
| Databáze: | OpenAIRE |
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