Chlamydia pneumoniaeInduces Interferon Gamma Responses in Peripheral Blood Mononuclear Cells in Children with Allergic Asthma

Autor:	Stephan Kohlhoff, Valeriy Chorny, Danielle Lent, Tamar A. Smith-Norowitz, Yitzchok M. Norowitz, Rauno Joks, Margaret R. Hammerschlag, Diana Weaver
Rok vydání:	2017
Předmět:	Male 0301 basic medicine Adolescent 030106 microbiology Immunology Enzyme-Linked Immunosorbent Assay Peripheral blood mononuclear cell Interferon-gamma 03 medical and health sciences 0302 clinical medicine Interferon Immunity Cell Line Tumor medicine Humans Interferon gamma Child Chlamydophila Infections Cells Cultured Asthma Chlamydia biology business.industry Respiratory infection General Medicine Chlamydophila pneumoniae medicine.disease Antibodies Bacterial respiratory tract diseases 030228 respiratory system Immunoglobulin G Leukocytes Mononuclear biology.protein Female Antibody business medicine.drug
Zdroj:	Scandinavian Journal of Immunology. 86:59-64
ISSN:	0300-9475
Popis:	Respiratory infections caused by Chlamydia pneumoniae have been associated with exacerbations of asthma. Cell mediated immunity (CMI) is critical for maintaining immunity. We compared Interferon (IFN)-γ responses in C. pneumoniae-infected peripheral blood mononuclear cells (PBMC) in pediatric patients +/- asthma. Presence of C. pneumoniae was tested from asthma patients (N=17) and non-asthmatic controls (N=16) (PCR). PBMC were infected for 1 hr +/- C. pneumoniae AR-39 (MOI = 0.1), and cultured for 48 hrs. IFN-γ levels were measured in supernatants (ELISA). C. pneumoniae-IgG antibodies in serum were determined (MIF). All subjects tested negative for C. pneumoniae (PCR). C. pneumoniae-induced IFN-γ production in vitro was more prevalent in asthma compared with non-asthma; levels of IFN-γ were higher in asthma compared with non-asthma (P= 0.003). There was no association between recent respiratory infection and positive IFN-γ responses. These data show that C. pneumoniae modulates IFN-γ responses in patients with asthma, even in absence of active infection. This article is protected by copyright. All rights reserved.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3286d6862a6d3e29ab1e0f762a76f47c https://doi.org/10.1111/sji.12561 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.