Sensitive colorimetric visualization of dihydronicotinamide adenine dinucleotide based on anti-aggregation of gold nanoparticles via boronic acid–diol binding
| Autor: | Peng Li, Shufeng Liu, Feng Li, Zongfeng Du |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Detection limit
Chemistry Diol Dihydronicotinamide Adenine Dinucleotide Biomedical Engineering Biophysics Metal Nanoparticles Biosensing Techniques General Medicine NAD Photochemistry Boronic Acids Sensitivity and Specificity Solvent chemistry.chemical_compound Linear range Limit of Detection Colloidal gold Electrochemistry Colorimetry Gold Sulfhydryl Compounds Naked eye Boronic acid Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 35:443-446 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2012.02.040 |
| Popis: | A facile, highly sensitive colorimetric strategy for dihydronicotinamide adenine dinucleotide (NADH) detection is proposed based on anti-aggregation of gold nanoparticles (AuNPs) via boronic acid-diol binding chemistry. The aggregation agent, 4-mercaptophenylboronic acid (MPBA), has specific affinity for AuNPs through Au-S interaction, leading to the aggregation of AuNPs by self-dehydration condensation at a certain concentration, which is responsible for a visible color change of AuNPs from wine red to blue. With the addition of NADH, MPBA would prefer reacting with NADH to form stable borate ester via boronic acid-diol binding dependent on the pH and solvent, revealing an obvious color change from blue to red with increasing the concentration of NADH. The anti-aggregation effect of NADH on AuNPs was seen by the naked eye and monitored by UV-vis extinction spectra. The linear range of the colorimetric sensor for NADH is from 8.0 × 10(-9)M to 8.0 × 10(-6)M, with a low detection limit of 2.0 nM. The as-established colorimetric strategy opened a new avenue for NADH determination. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect