Identification and cloning of a sequence homologue of dopamine β-hydroxylase
| Autor: | Maarten H.K. Linskens, Walter Funk, Kaylene J. Chambers, Leath A. Tonkin, Edwin Chang, Dawne N. Shelton |
|---|---|
| Přispěvatelé: | Groningen Biomolecular Sciences and Biotechnology |
| Rok vydání: | 1998 |
| Předmět: |
Signal peptide
DNA Complementary senescence Recombinant Fusion Proteins Molecular Sequence Data Gene Expression Dopamine beta-Hydroxylase Hybrid Cells Biology Mixed Function Oxygenases Mice Western blot Complementary DNA Genetics medicine Animals Humans Amino Acid Sequence RNA Messenger Cloning Molecular monooxygenase Gene Cells Cultured chemistry.chemical_classification Cloning Base Sequence medicine.diagnostic_test Chromosome Mapping General Medicine Monooxygenase CDNA chromosomal localization Fusion protein Molecular biology Enzyme chemistry Biochemistry Chromosomes Human Pair 6 |
| Zdroj: | Gene, 218(1). Elsevier |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/s0378-1119(98)00344-8 |
| Popis: | We have identified and cloned a cDNA encoding a new member of the monooxygenase family of enzymes. This novel enzyme, which we call MOX (monooxygenase X; unknown substrate) is a clear sequence homologue of the enzyme dopamine b-hydroxylase (DBH ). MOX maintains many of the structural features of DBH, as evidenced by the retention of most of the disulfide linkages and all of the peptidyl ligands to the active site copper atoms. Unlike DBH, MOX lacks a signal peptide sequence and therefore is unlikely to be a secreted molecule. The steady-state mRNA levels of MOX are highest in the kidney, lung, and adrenal gland, indicating that the tissue distribution of MOX is broader than that of DBH. Antisera raised to a fusion protein of MOX identifies a single band of the expected mobility by Western blot analysis. MOX mRNA levels are elevated in some fibroblast cell strains at replicative senescence, through this regulation is not apparent in all primary cell strains. The gene for MOX resides on the q arm of chromosome 6 and the corresponding mouse homolog has been identified. © 1998 Elsevier Science B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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