Human Mucosal Mast Cells Capture HIV-1 and Mediate Viral trans -Infection of CD4 + T Cells
| Autor: | Bao-Chi Liu, Yan Qin, Qian-Qian Guo, Ai-Ping Jiang, Ming-Gao Guo, Yong-Bing Ding, Ronald S. Veazey, Jin-Feng Jiang, Jian-Hua Wang, Xiaolei Wang, Ji-Fu Wei, Li Ma |
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| Rok vydání: | 2016 |
| Předmět: |
CD4-Positive T-Lymphocytes
0301 basic medicine Immunology Cell HIV Infections Microbiology 03 medical and health sciences Intestinal mucosa Virology Disease Transmission Infectious medicine Humans Mast Cells Intestinal Mucosa Cells Cultured Gastrointestinal tract biology Degranulation Mast cell Coculture Techniques Virus-Cell Interactions Mucosal Infection Interleukin 33 030104 developmental biology medicine.anatomical_structure Insect Science HIV-1 biology.protein Female Antibody |
| Zdroj: | Journal of Virology. 90:2928-2937 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | The gastrointestinal mucosa is the primary site where human immunodeficiency virus type 1 (HIV-1) invades, amplifies, and becomes persistently established, and cell-to-cell transmission of HIV-1 plays a pivotal role in mucosal viral dissemination. Mast cells are widely distributed in the gastrointestinal tract and are early targets for invasive pathogens, and they have been shown to have increased density in the genital mucosa in HIV-infected women. Intestinal mast cells express numerous pathogen-associated molecular patterns (PAMPs) and have been shown to combat various viral, parasitic, and bacterial infections. However, the role of mast cells in HIV-1 infection is poorly defined. In this study, we investigated their potential contributions to HIV-1 transmission. Mast cells isolated from gut mucosal tissues were found to express a variety of HIV-1 attachment factors (HAFs), such as DC-SIGN, heparan sulfate proteoglycan (HSPG), and α4β7 integrin, which mediate capture of HIV-1 on the cell surface. Intriguingly, following coculture with CD4 + T cells, mast cell surface-bound viruses were efficiently transferred to target T cells. Prior blocking with anti-HAF antibody or mannan before coculture impaired viral trans -infection. Cell-cell conjunctions formed between mast cells and T cells, to which viral particles were recruited, and these were required for efficient cell-to-cell HIV-1 transmission. Our results reveal a potential function of gut mucosal mast cells in HIV-1 dissemination in tissues. Strategies aimed at preventing viral capture and transfer mediated by mast cells could be beneficial in combating primary HIV-1 infection. IMPORTANCE In this study, we demonstrate the role of human mast cells isolated from mucosal tissues in mediating HIV-1 trans -infection of CD4 + T cells. This finding facilitates our understanding of HIV-1 mucosal infection and will benefit the development of strategies to combat primary HIV-1 dissemination. |
| Databáze: | OpenAIRE |
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