The effectiveness of the oncolytic activity induced by Ad5/F35 adenoviral vector is dependent on the cumulative cellular conditions of survival and autophagy
| Autor: | Joo Hang Kim, Jae J. Song, Sujin Kang, So Y. Kim |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Cancer Research
Cell Survival viruses Genetic Vectors bcl-X Protein Cytomegalovirus Biology medicine.disease_cause Adenoviridae Viral vector Membrane Cofactor Protein Transduction (genetics) Transduction Genetic Cell Line Tumor Autophagy Recoverin medicine Humans Promoter Regions Genetic Oncolytic Virotherapy Sirolimus Antibiotics Antineoplastic Oncogene Cell growth TOR Serine-Threonine Kinases Oncolytic virus Oncolytic Viruses Proto-Oncogene Proteins c-bcl-2 Oncology Cell culture Cancer cell Cancer research Proto-Oncogene Proteins c-akt |
| Zdroj: | International Journal of Oncology. 42:1337-1348 |
| ISSN: | 1791-2423 1019-6439 |
| Popis: | To overcome the poor tumor transduction efficiency of adenovirus serotype 5 (Ad5) observed in several types of cancer, the fiber region of Ad5, apart from its tail, was replaced by adenovirus serotype 35 (Ad35). The chimeric Ad5/F35 adenoviral vector did not exhibit any significant enhancement of transduction efficiency. CD46, a receptor for Ad35, was expressed in relatively small amounts in most of the cancer cells examined. Therefore, we investigated the pivotal factor(s) that render cancer cells susceptible to transduction. We discovered that the tumor transduction efficiency of Ad5/F35 was enhanced in the presence of rapamycin, an autophagy inducer, in some cancer cells. Analysis of survival potential and cell proliferation rates revealed that Ad5/F35 exerted a more pronounced oncolytic effect in cancer cells with higher survival potential in the presence of rapamycin. |
| Databáze: | OpenAIRE |
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