Light-induced retinal degeneration correlates with changes in iron metabolism gene expression, ferritin level, and aging
| Autor: | Jean-Claude Jeanny, Laurent Jonet, Isabelle Ranchon-Cole, Francine Behar-Cohen, Carole Beaumont, Yves Courtois, Emilie Picard |
|---|---|
| Přispěvatelé: | Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Clermont-Ferrand, Centre de recherche biomédicale Bichat-Beaujon (CRB3), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Picard, Emilie |
| Rok vydání: | 2010 |
| Předmět: |
Male
Aging Hephaestin Light [SDV]Life Sciences [q-bio] Ferroportin Mice 0302 clinical medicine MESH: Reverse Transcriptase Polymerase Chain Reaction Iron-Binding Proteins MESH: Aging MESH: Animals Fluorescent Antibody Technique Indirect Cation Transport Proteins chemistry.chemical_classification 0303 health sciences MESH: Iron Mice Inbred BALB C MESH: Oxidative Stress biology Reverse Transcriptase Polymerase Chain Reaction MESH: Retina Retinal Degeneration MESH: Enzyme-Linked Immunosorbent Assay Iron-binding proteins MESH: Gene Expression Regulation Cell biology [SDV] Life Sciences [q-bio] Radiation Injuries Experimental Female medicine.medical_specialty MESH: Retinal Degeneration Iron MESH: Mice Inbred BALB C Transferrin receptor Enzyme-Linked Immunosorbent Assay MESH: Apoferritins Retina 03 medical and health sciences MESH: Cation Transport Proteins Hepcidin Internal medicine medicine MESH: Fluorescent Antibody Technique Indirect Animals RNA Messenger MESH: Iron-Binding Proteins MESH: Mice 030304 developmental biology MESH: RNA Messenger MESH: Light MESH: Male Ferritin Oxidative Stress Endocrinology chemistry Gene Expression Regulation Transferrin MESH: Radiation Injuries Experimental Apoferritins 030221 ophthalmology & optometry biology.protein Ceruloplasmin MESH: Female |
| Zdroj: | Investigative Ophthalmology & Visual Science Investigative Ophthalmology & Visual Science, 2011, 52 (3), pp.1261. ⟨10.1167/iovs.10-5705⟩ Investigative Ophthalmology & Visual Science, Association for Research in Vision and Ophthalmology, 2011, 52 (3), pp.1261. ⟨10.1167/iovs.10-5705⟩ |
| ISSN: | 1552-5783 0146-0404 |
| Popis: | International audience; Purpose: Retinal degeneration is associated with iron accumulation in several rodent models in which iron-regulating proteins are impaired. Oxidative stress is catalyzed by unbound iron.Methods: The role of the heavy chain of ferritin, which sequesters iron, in regulating the thickness of the photoreceptor nuclear layer in the 4- and 16-month-old wild-type H ferritin (HFt(+/+)) and heterozygous H ferritin (HFt(+/-)) mice was investigated, before and 12 days after exposure to 13,000-lux light for 24 hours. The regulation of gene expression of the various proteins involved in iron homeostasis, such as transferrin, transferrin receptor, hephaestin, ferroportin, iron regulatory proteins 1 and 2, hepcidin, ceruloplasmin, and heme-oxygenase 1, was analyzed by quantitative (q)RT-PCR during exposure (2, 12, and 24 hours) and 24 hours after 1 day of exposure in the 4-month-old HFt(+/+) and HFt(+/-) mouse retinas.Results: Retinal degeneration in the 4-month-old HFt(+/-) mice was more extensive than in the HFt(+/+) mice. Yet, it was more extensive in both of the 16-month-old mouse groups, revealing the combined effect of age and excessive light. Injury caused by excessive light modified the temporal gene expression of iron-regulating proteins similarly in the HFt(+/-) and HFt(+/+) mice.Conclusions: Loss of one allele of H ferritin appears to increase light-induced degeneration. This study highlighted that oxidative stress related to light-induced injury is associated with major changes in gene expression of iron metabolism proteins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|