Analysis of Endothelial Adherence of Bartonella henselae and Acinetobacter baumannii Using a Dynamic Human Ex Vivo Infection Model
| Autor: | Volker Müller, Ralf P. Brandes, Stephan Göttig, Volkhard A. J. Kempf, Wibke Ballhorn, Doris Fischer, Sara Christ, Beate Averhoff, Dirk Linke, Gottfried Wilharm, Julia Stahl, Ju Ik Chae, Celestine Makobe, Marko Weidensdorfer, Christoph Schürmann |
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| Rok vydání: | 2016 |
| Předmět: |
Acinetobacter baumannii
0301 basic medicine 030106 microbiology Immunology Cellular microbiology Virulence In Vitro Techniques Microbiology Bacterial Adhesion Umbilical Cord 03 medical and health sciences In vivo Animals Humans Trimeric autotransporter adhesin Bartonella henselae biology Endothelial Cells Bacterial Infections biology.organism_classification Virology In vitro 030104 developmental biology Infectious Diseases Angiomatosis Bacillary Parasitology Ex vivo Acinetobacter Infections |
| Zdroj: | Infection and Immunity. 84:711-722 |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | Bacterial adherence determines the virulence of many human-pathogenic bacteria. Experimental approaches elucidating this early infection event in greater detail have been performed using mainly methods of cellular microbiology. However, in vitro infections of cell monolayers reflect the in vivo situation only partially, and animal infection models are not available for many human-pathogenic bacteria. Therefore, ex vivo infection of human organs might represent an attractive method to overcome these limitations. We infected whole human umbilical cords ex vivo with Bartonella henselae or Acinetobacter baumannii under dynamic flow conditions mimicking the in vivo infection situation of human endothelium. For this purpose, methods for quantifying endothelium-adherent wild-type and trimeric autotransporter adhesin (TAA)-deficient bacteria were set up. Data revealed that (i) A. baumannii binds in a TAA-dependent manner to endothelial cells, (ii) this organ infection model led to highly reproducible adherence rates, and furthermore, (iii) this model allowed to dissect the biological function of TAAs in the natural course of human infections. These findings indicate that infection models using ex vivo human tissue samples (“organ microbiology”) might be a valuable tool in analyzing bacterial pathogenicity with the capacity to replace animal infection models at least partially. |
| Databáze: | OpenAIRE |
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