Protein Phosphatase PP2C Identification in Entamoeba spp
| Autor: | Emiliano Tesoro-Cruz, Bibiana Chávez-Munguía, Alma Reyna Escalona-Montaño, Abril Navarrete-Mena, Verónica Ivonne Hernández-Ramírez, Patricia Talamás-Rohana, María Magdalena Aguirre-García, Jenny Nancy Gómez-Sandoval, Judith Pacheco-Yépez, Mario Nequiz-Avendaño |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Article Subject
Dispar Protozoan Proteins Biology General Biochemistry Genetics and Molecular Biology Entamoeba invadens Leishmania mexicana Microbiology Entamoeba Entamoeba histolytica fluids and secretions Western blot parasitic diseases medicine Parasite hosting Animals Humans Amoebiasis Amino Acid Sequence Trophozoites Phylogeny General Immunology and Microbiology medicine.diagnostic_test Entamoebiasis Sequence Homology Amino Acid General Medicine medicine.disease biology.organism_classification digestive system diseases Protein Phosphatase 2C Medicine Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2021 (2021) |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | Entamoeba histolytica is the causative agent of amoebiasis, and Entamoeba dispar is its noninvasive morphological twin. Entamoeba invadens is a reptilian parasite. In the present study, Western blot, phosphatase activity, immunofluorescence, and bioinformatic analyses were used to identify PP2C phosphatases of E. histolytica, E. dispar, and E. invadens. PP2C was identified in trophozoites of all Entamoeba species and cysts of E. invadens. Immunoblotting using a Leishmania mexicana anti-PP2C antibody recognized a 45.2 kDa PP2C in all species. In E. histolytica and E. invadens, a high molecular weight element PP2C at 75 kDa was recognized, mainly in cysts of E. invadens. Immunofluorescence demonstrated the presence of PP2C in membrane and vesicular structures in the cytosol of all species analyzed. The ~75 kDa PP2C of Entamoeba spp. shows the conserved domain characteristic of phosphatase enzymes (according to in silico analysis). Possible PP2C participation in the encystation process was discussed. |
| Databáze: | OpenAIRE |
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