A new approach to the phylogeny of Leishmania: species specificity of glycoconjugate ligands for promastigote internalization into murine macrophages
| Autor: | C. B. Palatnik, Radovan Borojevic, Lucia Mendonça-Previato, J. O. Previato |
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| Rok vydání: | 1990 |
| Předmět: |
Glycoconjugate
media_common.quotation_subject Phagocytosis Ligands Microbiology Mice Species Specificity parasitic diseases medicine Parasite hosting Animals Internalization Cells Cultured Phylogeny media_common chemistry.chemical_classification Leishmania General Veterinary biology Macrophages Leishmaniasis General Medicine medicine.disease biology.organism_classification Infectious Diseases Visceral leishmaniasis chemistry Insect Science Protozoa Parasitology Glycoconjugates |
| Zdroj: | Parasitology research. 76(4) |
| ISSN: | 0932-0113 |
| Popis: | Two Leishmania donovani glycoconjugate ligands for the internalization receptor on BALB/c peritoneal macrophages [fucose-mannose ligand (FML) and phosphate mannogalactan ligand (PMGL)] were shown to be species-specific in a comparative phagocytosis-inhibition test. Promastigotes of L. donovani Sudan (LD1S), L. infantum, L. d. donovani, L. major (Jericho and Sudan), L. tropica, L. chagasi, L. mexicana venezuelensis, L. m. mexicana, L. m. amazonensis, L. m. pifanoi, L. m. garnhami, L. braziliensis braziliensis, L. m. amazonensis (Josefa), L. enrietti or L. adleri were incubated with macrophages in the presence of 10 micrograms/ml FML and PMGL purified from L. donovani (LD1S). Parasite internalization was determined and compared with that obtained in control experiments. Specific inhibition of phagocytosis ranged from 83% (L. donovani LD1S) to 7% (L. m. amazonensis). We could distinguish groups of Leishmania consistently with their geographic distribution and the clinical aspects of the disease. Analogous experiments with L. m. amazonensis glycoconjugates showed reciprocal results, with inhibition ranging from 76% (L. m. amazonensis) to 8% (L. donovani LD1S). L. chagasi remained separated from the Old World kala-azar agents. Possible phylogenetic implications of these observations are discussed. |
| Databáze: | OpenAIRE |
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