Defining ELISpot cut-offs from unreplicated test and control wells

Autor: Le Quynh Mai, Nguyen Le Khanh Hang, Annette Fox, Neal Alexander, Vu Thi Kim Lien, Laurel Yong Hwa Lee, Peter Horby, Tao Dong
Rok vydání: 2013
Předmět:
Adult
PBMC
peripheral blood mononuclear cell
Enzyme-Linked Immunospot Assay
Standardized
Adolescent
Immunology
SFU
spot forming units
ELISpot
PHA
phytohemagglutinin
DMSO
dimethyl sulphoxide
ECDF
empirical cumulative distribution function
IAVI
International AIDS Vaccine Initiative
Cohort Studies
03 medical and health sciences
Young Adult
0302 clinical medicine
Statistics
Replication (statistics)
Influenza
Human
Technical Note
Medicine
Humans
Immunology and Allergy
030212 general & internal medicine
Child
030304 developmental biology
Aged
Aged
80 and over
0303 health sciences
ELISpot
enzyme-linked immunospot
Analysis of Variance
business.industry
ELISPOT
Cut-off
Middle Aged
3. Good health
Test (assessment)
ROC
receiver operating characteristic
Vietnam
Child
Preschool
Enzyme linked immunospot assay
business
Peptides
Zdroj: Journal of Immunological Methods; Vol 392
Journal of Immunological Methods
ISSN: 0022-1759
DOI: 10.1016/j.jim.2013.02.014
Popis: In the absence of replication of wells, empirical criteria for enzyme-linked immunospot (ELISpot) positivity use fixed differences or ratios between spot forming units (SFU) counts between test and control. We propose an alternative approach which first identifies the optimally variance-stabilizing transformation of the SFU counts, based on the Bland-Altman plot of the test and control wells. The second step is to derive a positivity threshold from the difference in between-plate distribution functions of the transformed test and control SFU counts. This method is illustrated using 1309 assay results from a cohort study of influenza in Vietnam in which some, but not all, of the peptide pools have clear tendencies for SFU counts to be higher in test than control wells. © 2013 Elsevier B.V.
Databáze: OpenAIRE
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